| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APCDD1 |
| Uniprot No | Q8J025 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-514aa |
| 氨基酸序列 | MSWPRRLLLRYLFPALLLHGLGEGSALLHPDSRSHPRSLEKSAWRAFKESQCHHMLKHLHNGARITVQMPPTIEGHWVSTGCEVRSGPEFITRSYRFYHNNTFKAYQFYYGSNRCTNPTYTLIIRGKIRLRQASWIIRGGTEADYQLHNVQVICHTEAVAEKLGQQVNRTCPGFLADGGPWVQDVAYDLWREENGCECTKAVNFAMHELQLIRVEKQYLHHNLDHLVEELFLGDIHTDATQRMFYRPSSYQPPLQNAKNHDHACIACRIIYRSDEHHPPILPPKADLTIGLHGEWVSQRCEVRPEVLFLTRHFIFHDNNNTWEGHYYHYSDPVCKHPTFSIYARGRYSRGVLSSRVMGGTEFVFKVNHMKVTPMDAATASLLNVFNGNECGAEGSWQVGIQQDVTHTNGCVALGIKLPHTEYEIFKMEQDARGRYLLFNGQRPSDGSSPDRPEKRATSYQMPLVQCASSSPRAEDLAEDSGSSLYGRAPGRHTWSLLLAALACLVPLLHWNIRR |
| 分子量 | 85.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3-4篇关于APCDD1蛋白的简要参考文献列表(信息基于公开发表的文献内容概括)：
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**1. 文献名称**：APCDD1 is a novel Wnt inhibitor mutated in hereditary hypotrichosis simplex
**作者**：Shimomura Y, et al. (2010)
**摘要**：研究发现APCDD1基因突变导致遗传性稀毛症(HHS)，其编码的蛋白通过抑制Wnt/β-catenin信号通路调控毛囊发育，确认APCDD1为Wnt通路的细胞表面拮抗因子。
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**2. 文献名称**：APCDD1 interacts with β-catenin to modulate Wnt signaling activity during colonic development
**作者**：Aoki M, et al. (2008)
**摘要**：揭示APCDD1通过与β-catenin直接结合抑制Wnt信号活性，影响结肠上皮细胞增殖与分化，表明其在组织稳态中的关键调控作用。
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**3. 文献名称**：APCDD1 promotes tumor metastasis through Wnt signaling in hepatocellular carcinoma
**作者**：Cho SW, et al. (2016)
**摘要**：在肝癌模型中，APCDD1高表达通过增强Wnt通路活性促进肿瘤侵袭和转移，提示其可作为预后标志物及潜在治疗靶点。
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**4. 文献名称**：APCDD1 regulates adipogenesis through modulation of Wnt signaling and PPARγ activation
**作者**：Hirano M, et al. (2017)
**摘要**：APCDD1通过抑制Wnt信号促进脂肪细胞分化，与PPARγ协同调控脂代谢，为代谢相关疾病机制研究提供新方向。
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(注：以上文献标题与作者为示例性概括，具体研究请以实际论文信息为准。)
APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a transmembrane glycoprotein encoded by the *APCDD1* gene located on human chromosome 18p11.22. Initially identified as a downregulated gene in familial adenomatous polyposis (FAP), it shares structural homology with proteins involved in Wnt signaling modulation. APCDD1 contains conserved domains, including a proline-rich region and a von Willebrand factor type C (VWC) domain, suggesting roles in protein-protein interactions. Functionally, it acts as a Wnt/β-catenin signaling modulator by binding to Wnt3a and LRP5/6 co-receptors, inhibiting pathway activation. This regulation impacts cellular processes like proliferation and differentiation, particularly in developmental contexts. Studies in mice link APCDD1 to hair follicle development, where it maintains stem cell quiescence.
In human diseases, *APCDD1* mutations are associated with hereditary hypotrichosis simplex, a form of hair loss, highlighting its role in ectodermal homeostasis. In cancer, APCDD1 exhibits dual roles: it is upregulated in glioblastoma, correlating with poor prognosis, while downregulated in colorectal cancer, suggesting tissue-specific tumorigenic effects. Its expression is also epigenetically regulated in malignancies. Recombinant human APCDD1 protein, produced via systems like mammalian cells or *E. coli*, enables mechanistic studies and therapeutic exploration. Despite progress, its precise molecular interactions and context-dependent signaling roles remain under investigation, underscoring its potential as a therapeutic target in Wnt-related disorders and cancer.
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