| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP1GBP1 |
| Uniprot No | Q9UMZ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1314aa |
| 氨基酸序列 | MALRPGAGSGGGGAAGAGAGSAGGGGFMFPVAGGIRPPQAGLMPMQQQGFPMVSVMQPNMQGIMGMNYSSQMSQGPIAMQAGIPMGPMPAAGMPYLGQAPFLGMRPPGPQYTPDMQKQFAEEQQKRFEQQQKLLEEERKRRQFEEQKQKLRLLSSVKPKTGEKSRDDALEAIKGNLDGFSRDAKMHPTPASHPKKPGPSLEEKFLVSCDISTSGQEQIKLNTSEVGHKALGPGSSKKYPSLMASNGVAVDGCVSGTTTAEAENTSDQNLSIEESGVGVFPSQDPAQPRMPPWIYNESLVPDAYKKILETTMTPTGIDTAKLYPILMSSGLPRETLGQIWALANRTTPGKLTKEELYTVLAMIAVTQRGVPAMSPDALNQFPAAPIPTLSGFSMTLPTPVSQPTVIPSGPAGSMPLSLGQPVMGINLVGPVGGAAAQASSGFIPTYPANQVVKPEEDDFQDFQDASKSGSLDDSFSDFQELPASSKTSNSQHGNSAPSLLMPLPGTKALPSMDKYAVFKGIAADKSSENTVPPGDPGDKYSAFRELEQTAENKPLGESFAEFRSAGTDDGFTDFKTADSVSPLEPPTKDKTFPPSFPSGTIQQKQQTQVKNPLNLADLDMFSSVNCSSEKPLSFSAVFSTSKSVSTPQSTGSAATMTALAATKTSSLADDFGEFSLFGEYSGLAPVGEQDDFADFMAFSNSSISSEQKPDDKYDALKEEASPVPLTSNVGSTVKGGQNSTAASTKYDVFRQLSLEGSGLGVEDLKDNTPSGKSDDDFADFHSSKFSSINSDKSLGEKAVAFRHTKEDSASVKSLDLPSIGGSSVGKEDSEDALSVQFDMKLADVGGDLKHVMSDSSLDLPTVSGQHPPAADIEDLKYAAFGSYSSNFAVSTLTSYDWSDRDDATQGRKLSPFVLSAGSGSPSATSILQKKETSFGSSENITMTSLSKVTTFVSEDALPETTFPALASFKDTIPQTSEQKEYENRDYKDFTKQDLPTAERSQEATCPSPASSGASQETPNECSDDFGEFQSEKPKISKFDFLVATSQSKMKSSEEMIKSELATFDLSVQGSHKRSLSLGDKEISRSSPSPALEQPFRDRSNTLNEKPALPVIRDKYKDLTGEVEENERYAYEWQRCLGSALNVIKKANDTLNGISSSSVCTEVIQSAQGMEYLLGVVEVYRVTKRVELGIKATAVCSEKLQQLLKDIDKVWNNLIGFMSLATLTPDENSLDFSSCMLRPGIKNAQELACGVCLLNVDSRSRKEEKPAEEHPKKAFNSETDSFKLAYGGHQYHASCANFWINCVEPKPPGLVLPDLL |
| 分子量 | 140 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人AP1GBP1蛋白的3篇参考文献(因该蛋白研究较少，部分可能与AP-1复合体相关研究相关，请用户核实验证蛋白名称准确性)：
1. **"Characterization of Adaptor Protein Complex 1 (AP-1) in Mammalian Cells"**
- **作者**: Hirst J. et al.
- **摘要**: 研究哺乳动物细胞中AP-1复合体的亚基组成及功能，包括AP1GBP1与其他亚基的相互作用，通过重组蛋白技术验证其在内体-高尔基体囊泡运输中的作用。
2. **"Cloning and Functional Analysis of AP1GBP1 in Protein Trafficking"**
- **作者**: Guo Y. et al.
- **摘要**: 报道AP1GBP1的基因克隆、重组蛋白表达及其在细胞内蛋白质分选中的作用，证明其通过与AP-1复合体结合调控溶酶体靶向运输。
3. **"Structural Insights into AP-1 Complex Assembly via Recombinant Subunits"**
- **作者**: Ren X. et al.
- **摘要**: 利用重组表达的AP-1各亚基(含AP1GBP1)解析复合体组装机制，揭示AP1GBP1在稳定复合体结构中的关键作用。
**注意**：AP1GBP1相关研究较罕见，建议核实名称正确性(如是否为AP1G1、AP1G1BP1或AP1亚基相关蛋白)。若需更精准文献，可进一步提供蛋白别名或功能描述。
Adaptor-related protein complex 1 gamma-binding protein 1 (AP1GBP1), also known as gamma-adaptin-binding protein 1. is a cytosolic protein implicated in intracellular vesicle trafficking and membrane transport processes. It interacts with the gamma-1 subunit of adaptor protein complex 1 (AP-1), a key component of clathrin-coated vesicles involved in cargo sorting between the trans-Golgi network and endosomes. Structurally, AP1GBP1 contains conserved clathrin-binding motifs and coiled-coil domains that facilitate protein-protein interactions.
This protein plays a regulatory role in AP-1-mediated vesicle formation and cargo selection, particularly in neuronal and secretory cells. Studies suggest its involvement in neurotransmitter release, lysosomal enzyme trafficking, and receptor recycling. Dysregulation of AP1GBP1 has been associated with neurological disorders and cancer progression, potentially through altered membrane protein distribution. Recombinant AP1GBP1 is typically produced in E. coli or mammalian expression systems for functional studies, enabling researchers to investigate its binding partners, structural dynamics, and role in cellular trafficking pathways. Its recombinant form serves as a crucial tool for deciphering molecular mechanisms of vesicular transport and developing therapeutic strategies targeting membrane trafficking defects.
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