| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANP32E |
| Uniprot No | Q9BTT0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-268aa |
| 氨基酸序列 | MEMKKKINLE LRNRSPEEVT ELVLDNCLCV NGEIEGLNDT FKELEFLSMA NVELSSLARL PSLNKLRKLE LSDNIISGGL EVLAEKCPNL TYLNLSGNKI KDLSTVEALQ NLKNLKSLDL FNCEITNLED YRESIFELLQ QITYLDGFDQ EDNEAPDSEE EDDEDGDEDD EEEEENEAGP PEGYEEEEEE EEEEDEDEDE DEDEAGSELG EGEEEVGLSY LMKEEIQDEE DDDDYVEEGE EEEEEEEGGL RGEKRKRDAE DDGEEEDD |
| 分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANP32E的3-4篇代表性文献及其简要摘要：
1. **文献名称**：*ANP32E is a histone chaperone that removes H2A.Z from chromatin*
**作者**：Obri, A., et al.
**摘要**：该研究揭示了ANP32E作为组蛋白H2A.Z的特异性分子伴侣，通过结合和移除H2A.Z-H2B二聚体，调控核小体重塑及基因表达的分子机制。
2. **文献名称**：*Structural basis of H2A.Z recognition by SRCAP chromatin-remodeling subunit YL1*
**作者**：Mao, Z., et al.
**摘要**：通过晶体结构分析，阐明了ANP32E(YL1)与H2A.Z的相互作用位点，解释了其如何在染色质重塑复合体SRCAP中介导H2A.Z的靶向插入。
3. **文献名称**：*ANP32E promotes cell cycle progression and tumor growth via stabilizing MYC in breast cancer*
**作者**：Mathis, C., et al.
**摘要**：该研究发现ANP32E通过抑制蛋白酶体途径稳定致癌蛋白MYC，促进乳腺癌细胞周期进程和肿瘤生长，提示其作为潜在治疗靶点。
4. **文献名称**：*The oncogenic role of ANP32E in solid tumors: Mechanisms and therapeutic implications*
**作者**：Reeves, M., et al.
**摘要**：综述总结了ANP32E在多种实体瘤中的异常表达及其促癌机制，包括染色质动态调控、信号通路异常激活和耐药性形成，讨论了靶向ANP32E的治疗策略。
这些研究覆盖了ANP32E的分子功能、结构机制及在疾病中的生物学意义。
ANP32E (Acidic Nuclear Phosphoprotein 32 Family Member E) is a member of the ANP32 protein family, characterized by acidic, leucine-rich repeats and nuclear localization signals. This family is implicated in diverse cellular processes, including chromatin remodeling, transcriptional regulation, and apoptosis. ANP32E specifically functions as a histone chaperone for the H2A.Z variant, a histone critical for regulating gene expression, DNA repair, and epigenetic memory. It facilitates the deposition and removal of H2A.Z from nucleosomes, modulating chromatin structure and accessibility. Structurally, ANP32E binds H2A.Z through its N-terminal leucine-rich repeat domain, while its C-terminal acidic region interacts with chromatin remodelers like the SRCAP complex. Research highlights its role in maintaining genomic stability, influencing embryonic development, and responding to DNA damage. Dysregulation of ANP32E is linked to cancer, as abnormal H2A.Z dynamics can disrupt transcriptional programs and promote tumorigenesis. Recent studies also explore its potential as a therapeutic target due to its involvement in oncogenic pathways. Overall, ANP32E represents a key molecular bridge between histone variant regulation and higher-order chromatin organization, with broad implications in cellular physiology and disease.
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