| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANP32B |
| Uniprot No | Q92688 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-251aa |
| 氨基酸序列 | MDMKRRIHLE LRNRTPAAVR ELVLDNCKSN DGKIEGLTAE FVNLEFLSLI NVGLISVSNL PKLPKLKKLE LSENRIFGGL DMLAEKLPNL THLNLSGNKL KDISTLEPLK KLECLKSLDL FNCEVTNLND YRESVFKLLP QLTYLDGYDR EDQEAPDSDA EVDGVDEEEE DEEGEDEEDE DDEDGEEEEF DEEDDEDEDV EGDEDDDEVS EEEEEFGLDE EDEDEDEDEE EEEGGKGEKR KRETDDEGED D |
| 分子量 | 28.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANP32B的3-4篇文献示例(内容为示例性概括，非真实文献)：
1. **文献名称**：*ANP32B promotes acute myeloid leukemia progression by regulating oncogenic signaling pathways*
**作者**：Rehan M, et al.
**摘要**：该研究揭示了ANP32B在急性髓系白血病(AML)中的关键作用，其通过调控Wnt/β-catenin和NF-κB通路促进白血病细胞增殖，并抑制凋亡。基因敲除ANP32B显著延缓了小鼠模型中AML的进展。
2. **文献名称**：*ANP32B supports influenza virus replication by stabilizing viral RNA polymerase*
**作者**：Sugiyama K, et al.
**摘要**：研究证明ANP32B可作为宿主因子支持甲型流感病毒的RNA聚合酶活性，尤其在ANP32A缺失的哺乳动物细胞中，ANP32B对病毒基因组的转录和复制起到补偿作用。
3. **文献名称**：*ANP32B acts as a scaffold protein in Wnt/β-catenin signaling to drive colorectal cancer*
**作者**：Zhang Y, et al.
**摘要**：ANP32B通过结合β-catenin和核转运蛋白，促进β-catenin的核定位并增强其转录活性，进而驱动结直肠癌细胞的侵袭和转移。靶向ANP32B可抑制小鼠肿瘤模型中的转移。
4. **文献名称**：*ANP32B deficiency leads to embryonic lethality via dysregulation of Hox gene expression*
**作者**：Seo H, et al.
**摘要**：研究发现ANP32B基因敲除小鼠胚胎因发育异常而致死，其机制与Hox基因表达失调和细胞周期调控异常相关，提示ANP32B在早期胚胎发育中的关键作用。
(注：以上文献为示例性描述，实际研究中请通过PubMed等数据库检索具体文献。)
ANP32B (Acidic Leucine-Rich Nuclear Phosphoprotein 32 Family Member B), also known as APRIL or PHAPI2. belongs to the ANP32 family of proteins involved in diverse cellular processes including chromatin remodeling, transcriptional regulation, apoptosis, and signal transduction. This highly conserved protein is characterized by an N-terminal leucine-rich repeat (LRR) domain and an acidic C-terminal region. ANP32B localizes predominantly to the nucleus, where it interacts with histones, nuclear transport factors, and chromatin-modifying complexes. It functions as a molecular chaperone or co-regulator, influencing epigenetic regulation by suppressing histone acetylation through interactions with histone deacetylases (HDACs) or inhibiting histone acetyltransferases (HATs).
ANP32B also plays roles in cancer progression, viral infections (e.g., supporting influenza viral RNA polymerase activity), and neuronal development. Its involvement in pathological conditions includes neurodegenerative diseases and tumorigenesis, though precise mechanisms remain under investigation. Studies highlight its dual roles in promoting cell survival and apoptosis, depending on cellular context. Notably, ANP32B knockout mice exhibit developmental defects, emphasizing its biological importance. Recent research focuses on its potential as a therapeutic target, particularly in oncology and antiviral strategies.
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