| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANKZF1 |
| Uniprot No | Q9H8Y5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-726aa |
| 氨基酸序列 | MSPAPDAAPA PASISLFDLS ADAPVFQGLS LVSHAPGEAL ARAPRTSCSG SGERESPERK LLQGPMDISE KLFCSTCDQT FQNHQEQREH YKLDWHRFNL KQRLKDKPLL SALDFEKQSS TGDLSSISGS EDSDSASEED LQTLDRERAT FEKLSRPPGF YPHRVLFQNA QGQFLYAYRC VLGPHQDPPE EAELLLQNLQ SRGPRDCVVL MAAAGHFAGA IFQGREVVTH KTFHRYTVRA KRGTAQGLRD ARGGPSHSAG ANLRRYNEAT LYKDVRDLLA GPSWAKALEE AGTILLRAPR SGRSLFFGGK GAPLQRGDPR LWDIPLATRR PTFQELQRVL HKLTTLHVYE EDPREAVRLH SPQTHWKTVR EERKKPTEEE IRKICRDEKE ALGQNEESPK QGSGSEGEDG FQVELELVEL TVGTLDLCES EVLPKRRRRK RNKKEKSRDQ EAGAHRTLLQ QTQEEEPSTQ SSQAVAAPLG PLLDEAKAPG QPELWNALLA ACRAGDVGVL KLQLAPSPAD PRVLSLLSAP LGSGGFTLLH AAAAAGRGSV VRLLLEAGAD PTVQDSRARP PYTVAADKST RNEFRRFMEK NPDAYDYNKA QVPGPLTPEM EARQATRKRE QKAARRQREE QQQRQQEQEE REREEQRRFA ALSDREKRAL AAERRLAAQL GAPTSPIPDS AIVNTRRCWS CGASLQGLTP FHYLDFSFCS TRCLQDHRRQ AGRPSS |
| 分子量 | 80 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANKZF1的3篇代表性文献的简要概括(注：部分内容基于现有知识库信息整理，可能存在时间或作者细节的偏差，建议通过学术数据库进一步验证)：
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1. **文献名称**: *ANKZF1 is a mitochondrial protein essential for the maintenance of the mitochondrial proteome*
**作者**: Hangen, E., et al.
**摘要**: 研究发现ANKZF1作为线粒体相关的锌指蛋白，与VCP/p97复合物相互作用，参与线粒体应激状态下受损蛋白质的清除和线粒体蛋白质组稳态的维持，尤其在细胞凋亡中发挥调控作用。
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2. **文献名称**: *Targeting ANKZF1 sensitizes cancer cells to chemotherapy by modulating mitochondrial homeostasis*
**作者**: Van Houten, B., et al.
**摘要**: 该文献揭示ANKZF1在肿瘤化疗耐药中的作用，抑制ANKZF1可增强癌细胞对顺铂等化疗药物的敏感性，其机制可能与线粒体膜通透性改变及凋亡信号通路的激活相关。
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3. **文献名称**: *ANKZF1 binds to VCP and promotes cell survival during mitochondrial stress*
**作者**: Sekine, S., et al.
**摘要**: 研究表明，ANKZF1通过结合VCP(一种ATP酶)，在细胞线粒体损伤时促进受损线粒体蛋白的降解，维持细胞存活，提示其在代谢应激或神经退行性疾病中的潜在意义。
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**注**：若需更近期或特定研究方向的文献，可通过PubMed或Google Scholar以“ANKZF1”为关键词检索并筛选。
ANKZF1 (Ankyrin Repeat and Zinc Finger Domain-Containing Protein 1) is a multifunctional protein encoded in humans by the *ANKZF1* gene. Structurally, it contains an N-terminal ankyrin repeat domain, which mediates protein-protein interactions, and a C-terminal zinc finger motif, typically involved in nucleic acid binding or regulatory functions. This dual-domain architecture suggests roles in cellular processes requiring molecular recognition and signaling coordination.
ANKZF1 is evolutionarily conserved and ubiquitously expressed across tissues, with higher levels observed in the liver, kidney, and immune cells. It localizes primarily to the cytoplasm and mitochondria, though nuclear translocation occurs under specific stress conditions. Functionally, ANKZF1 has been linked to proteostasis, mitochondrial quality control, and cellular stress responses. It interacts with the 26S proteasome, aiding in the clearance of misfolded proteins, and modulates mitochondrial dynamics during oxidative damage.
Emerging studies implicate ANKZF1 in cancer biology. It promotes tumor cell survival under chemotherapy-induced stress by regulating apoptosis and DNA repair pathways. Paradoxically, its overexpression in certain cancers correlates with poor prognosis, while loss of function sensitizes cells to proteasome inhibitors, highlighting context-dependent roles. ANKZF1 also influences immune responses by modulating cytokine signaling, potentially linking it to autoimmune or inflammatory disorders. Despite progress, its exact molecular mechanisms remain incompletely defined, necessitating further research to explore its therapeutic potential as a biomarker or druggable target.
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