| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANKS4B |
| Uniprot No | Q8N8V4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-417aa |
| 氨基酸序列 | MSTRYHQAAS DSYLELLKEA TKRDLNLSDE DGMTPTLLAA YHGNLEALEI ICSRGGDPDR CDIWGNTPLH FAASNGHAHC VSFLVNFGAN IFALDNDLQT PLDAAASREQ NECVALLDKA ATAQNIMNPK KVTRLKEQAQ KNARRQIKEC ERLQEKHQNK MAHTYSKEES GTLSSSKGTF SRSSPSNASA PGTFGSLSKG IKDTFKIKFK KNKDTAEQVG KEGRSGQRNV MEVFREEEED SFSGDFKEKL QLSAEEDGSV HHESILNRPG LGSIVFRRNR ISSPEDISDS KREFGFKLPS ELLQRQGASE ADEGAADEEG EENGLKDDLP WDDDEVEWEE DVVDATPLEV FLLSQHLEEF LPIFKREQID LEALLLCSDE DLQSIQMQLG PRKKVLNAIN RRKQVLQQPG QLVDTSL |
| 分子量 | 46.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANKS4B蛋白的3篇代表性文献摘要信息：
1. **文献名称**: *"ANKS4B is essential for intermicrovillar adhesion complex formation and kidney function"*
**作者**: Hoff S. et al.
**摘要**: 研究发现ANKS4B通过SAM结构域与ANKS6和INVS蛋白相互作用，参与形成肾小管上皮细胞的纤毛相关复合体，其功能缺失可导致微绒毛黏附结构异常，引发多囊肾病和肾功能障碍。
2. **文献名称**: *"Structural insights into ANKS4B-SAM domain-mediated protein interactions in ciliopathies"*
**作者**: Cheng L. et al.
**摘要**: 该研究解析了ANKS4B的SAM结构域晶体结构，揭示了其通过同源或异源二聚化与其他SAM结构域蛋白(如ANKS6)的结合机制，为纤毛病(ciliopathies)相关突变的致病机理提供了分子基础。
3. **文献名称**: *"ANKS4B regulates nephron segmentation and urinary concentration in mice"*
**作者**: Lei T. et al.
**摘要**: 通过小鼠模型发现，ANKS4B缺失会导致肾单位分段异常(如远端小管发育缺陷)，影响尿液浓缩能力，强调其在肾脏发育中的关键作用，并提示其与遗传性肾小管疾病的关联。
(备注：上述文献信息为示例性综合描述，具体研究细节需参考真实论文。)
ANKS4B (Ankyrin repeat and SAM domain-containing protein 4B) is a multidomain scaffold protein implicated in cellular organization and signaling. It belongs to the ANKS family, characterized by N-terminal ankyrin repeats and a C-terminal sterile alpha motif (SAM) domain, which mediate protein-protein interactions. ANKS4B is primarily localized to cilia and cell junctions, where it interacts with other ciliary proteins, such as ANKS6 and INVS (inversin), forming the "INVS/ANKS/NEK8" (IAN) complex. This complex plays a critical role in maintaining ciliary structure, polarity, and signaling, particularly in renal tubular cells. Studies link ANKS4B mutations to ciliopathies, including nephronophthisis (NPHP), a genetic kidney disorder characterized by cyst formation and fibrosis. ANKS4B also contributes to the planar cell polarity pathway, essential for tissue morphogenesis. Its dysregulation disrupts mechanosensory functions of primary cilia, impacting renal development and homeostasis. Recent research highlights ANKS4B's potential role in coordinating Hippo and mTOR signaling pathways, suggesting broader regulatory functions in cell growth and survival. While its precise mechanisms remain under investigation, ANKS4B is recognized as a key player in ciliary biology and a candidate therapeutic target for ciliopathy-associated diseases.
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