| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APOBEC3F |
| Uniprot No | Q8IUX4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-373aa |
| 氨基酸序列 | MKPHFRNTVE RMYRDTFSYN FYNRPILSRR NTVWLCYEVK TKGPSRPRLD AKIFRGQVYS QPEHHAEMCF LSWFCGNQLP AYKCFQITWF VSWTPCPDCV AKLAEFLAEH PNVTLTISAA RLYYYWERDY RRALCRLSQA GARVKIMDDE EFAYCWENFV YSEGQPFMPW YKFDDNYAFL HRTLKEILRN PMEAMYPHIF YFHFKNLRKA YGRNESWLCF TMEVVKHHSP VSWKRGVFRN QVDPETHCHA ERCFLSWFCD DILSPNTNYE VTWYTSWSPC PECAGEVAEF LARHSNVNLT IFTARLYYFW DTDYQEGLRS LSQEGASVEI MGYKDFKYCW ENFVYNDDEP FKPWKGLKYN FLFLDSKLQE ILE |
| 分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人APOBEC3F蛋白的模拟参考文献示例(实际文献需通过学术数据库检索)：
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1. **文献名称**：*APOBEC3F-mediated antiviral mechanisms against HIV-1 and functional interplay with Vif*
**作者**：Smith JL et al.
**摘要**：揭示了APOBEC3F通过诱导病毒DNA超突变抑制HIV-1复制，并证明HIV-1 Vif蛋白通过招募E3泛素连接酶复合体降解APOBEC3F，从而逃逸宿主防御。
2. **文献名称**：*Biochemical characterization of recombinant human APOBEC3F deaminase activity*
**作者**：Zhang Y et al.
**摘要**：报道了重组APOBEC3F的体外表达与纯化方法，证实其优先靶向单链DNA中TC序列的胞嘧啶，通过酶动力学实验量化其催化效率，为药物开发提供依据。
3. **文献名称**：*Structural insights into APOBEC3F substrate specificity and inhibition by HIV-1 Vif*
**作者**：Wang X et al.
**摘要**：利用X射线晶体学解析APOBEC3F结构，识别与DNA结合及脱氨活性相关的关键结构域，并阐明Vif通过特定界面结合抑制其功能的分子机制。
4. **文献名称**：*APOBEC3F overexpression promotes tumor mutagenesis in human hepatocellular carcinoma*
**作者**：Li H et al.
**摘要**：临床研究发现肝癌组织中APOBEC3F异常高表达，体外实验表明其过表达导致宿主基因组DNA突变率增加，提示其在癌症发生中的双重角色。
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**提示**：以上为模拟摘要，实际文献请通过**PubMed**、**Google Scholar**等平台检索，关键词建议：
- "recombinant APOBEC3F purification"
- "APOBEC3F HIV restriction"
- "APOBEC3F structural analysis"
Recombinant human APOBEC3F (Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 3F) is a cytidine deaminase protein belonging to the APOBEC3 family, which plays a critical role in innate antiviral immunity. APOBEC3 proteins are renowned for their ability to restrict retroviruses, including HIV-1. by inducing hypermutations in viral genomes. APOBEC3F, specifically, is expressed in immune cells and certain tissues, acting as a host defense factor by catalyzing the deamination of cytosine to uracil in single-stranded DNA. This activity generates lethal mutations during viral reverse transcription, impairing viral replication.
Structurally, APOBEC3F contains two conserved zinc-binding domains, with the C-terminal domain primarily driving its antiviral activity. Recombinant APOBEC3F is typically produced in mammalian expression systems (e.g., HEK293 cells) to ensure proper folding and post-translational modifications. Research highlights its synergistic role with APOBEC3G in countering HIV-1. though HIV counteracts this defense via the Vif protein, which targets APOBEC3F for proteasomal degradation.
Beyond antiviral functions, APOBEC3F is implicated in cancer mutagenesis due to off-target DNA editing. Studies focus on its biochemical properties, regulation, and interactions to develop therapeutic strategies against viral infections or modulate its activity in genome-editing applications. Its dual role as a protector and potential mutagen underscores its biological complexity.
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