| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARCN1 |
| Uniprot No | P48444 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-511aa |
| 氨基酸序列 | MVLLAAAVCT KAGKAIVSRQ FVEMTRTRIE GLLAAFPKLM NTGKQHTFVE TESVRYVYQP MEKLYMVLIT TKNSNILEDL ETLRLFSRVI PEYCRALEEN EISEHCFDLI FAFDEIVALG YRENVNLAQI RTFTEMDSHE EKVFRAVRET QEREAKAEMR RKAKELQQAR RDAERQGKKA PGFGGFGSSA VSGGSTAAMI TETIIETDKP KVAPAPARPS GPSKALKLGA KGKEVDNFVD KLKSEGETIM SSSMGKRTSE ATKMHAPPIN MESVHMKIEE KITLTCGRDG GLQNMELHGM IMLRISDDKY GRIRLHVENE DKKGVQLQTH PNVDKKLFTA ESLIGLKNPE KSFPVNSDVG VLKWRLQTTE ESFIPLTINC WPSESGNGCD VNIEYELQED NLELNDVVIT IPLPSGVGAP VIGEIDGEYR HDSRRNTLEW CLPVIDAKNK SGSLEFSIAG QPNDFFPVQV SFVSKKNYCN IQVTKVTQVD GNSPVRFSTE TTFLVDKYEI L |
| 分子量 | 83.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COPⅠ蛋白亚基delta(ARCN1)的3篇代表性文献摘要列举(注：文献为虚拟示例，仅供参考框架)：
1. **"Structural Insights into the COPⅠ Delta Subunit ARCN1 in Vesicle Coat Assembly"**
- **作者**：Chou H.T., et al.
- **摘要**：通过冷冻电镜解析ARCN1的分子结构，揭示其与其他COPI亚基的相互作用机制，并阐明其在COPⅠ复合体囊泡组装中的关键结构功能。
2. **"ARCN1 Mutations Disrupt ER-Golgi Trafficking and Cause Craniofacial Dysplasia"**
- **作者**：Ogunleye E.T., et al.
- **摘要**：首次发现ARCN1基因突变与先天性骨骼发育异常相关，实验表明突变导致COPI复合体功能缺陷，影响内质网-高尔基体逆向运输，引发细胞分泌途径异常。
3. **"Functional Dissection of ARCN1 in COPI-Mediated Membrane Transport"**
- **作者**：Garabedian D.S., et al.
- **摘要**：利用敲除细胞模型，证实ARCN1对COPI囊泡形成至关重要，其缺失导致溶酶体酶错误分选，并破坏细胞内脂质稳态。
(注：以上文献及作者为示例性质，实际文献需通过PubMed/Google Scholar等平台按关键词检索后引用。)
**Background of Human COPI Protein Subunit Delta (ARCN1)**
The Coatomer Protein Complex I (COPI) is a conserved multisubunit complex essential for intracellular vesicular trafficking, primarily mediating retrograde transport from the Golgi apparatus to the endoplasmic reticulum (ER) and within Golgi cisternae. ARCN1 (δ-COP), encoded by the *ARCN1* gene, is a critical subunit of the COPI complex. Structurally, COPI comprises seven subunits (α to ζ), with ARCN1 contributing to the stability and functional assembly of the complex.
ARCN1 plays a role in cargo recognition and coatomer binding to membranes, facilitating vesicle formation and cargo sorting. It interacts with components like the KDEL receptor, ensuring retrieval of ER-resident proteins. Dysregulation of ARCN1 disrupts COPI function, impairing cellular processes such as protein secretion, organelle maintenance, and glycosylation.
Beyond its trafficking role, ARCN1 is linked to developmental and pathological conditions. Mutations in *ARCN1* are associated with skeletal dysplasia and craniofacial abnormalities, highlighting its importance in development. Additionally, altered ARCN1 expression is observed in cancers, influencing tumor proliferation and metastasis. Its dual roles in fundamental trafficking and disease underscore ARCN1 as a vital research target in cell biology and medicine.
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