| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APPL2 |
| Uniprot No | Q8NEU8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-664aa |
| 氨基酸序列 | MPAVDKLLLE EALQDSPQTR SLLSVFEEDA GTLTDYTNQL LQAMQRVYGA QNEMCLATQQ LSKQLLAYEK QNFALGKGDE EVISTLHYFS KVVDELNLLH TELAKQLADT MVLPIIQFRE KDLTEVSTLK DLFGLASNEH DLSMAKYSRL PKKKENEKVK TEVGKEVAAA RRKQHLSSLQ YYCALNALQY RKQMAMMEPM IGFAHGQINF FKKGAEMFSK RMDSFLSSVA DMVQSIQVEL EAEAEKMRVS QQELLSVDES VYTPDSDVAA PQINRNLIQK AGYLNLRNKT GLVTTTWERL YFFTQGGNLM CQPRGAVAGG LIQDLDNCSV MAVDCEDRRY CFQITTPNGK SGIILQAESR KENEEWICAI NNISRQIYLT DNPEAVAIKL NQTALQAVTP ITSFGKKQES SCPSQNLKNS EMENENDKIV PKATASLPEA EELIAPGTPI QFDIVLPATE FLDQNRGSRR TNPFGETEDE SFPEAEDSLL QQMFIVRFLG SMAVKTDSTT EVIYEAMRQV LAARAIHNIF RMTESHLMVT SQSLRLIDPQ TQVSRANFEL TSVTQFAAHQ ENKRLVGFVI RVPESTGEES LSTYIFESNS EGEKICYAIN LGKEIIEVQK DPEALAQLML SIPLTNDGKY VLLNDQPDDD DGNPNEHRGA ESEA |
| 分子量 | 74 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DCC相互作用蛋白13-β(APPL2)的3篇代表性文献：
1. **文献名称**: *APPL2 interacts with the adiponectin receptor 2 to regulate metabolic signaling*
**作者**: Min, C., et al. (2009)
**摘要**: 该研究揭示了APPL2通过与脂联素受体2结合，在胰岛素信号通路中发挥调节作用，影响葡萄糖代谢和线粒体功能。
2. **文献名称**: *APPL1 and APPL2 are regulators of PI3K signaling and insulin sensitivity in mouse models*
**作者**: Schenck, A., et al. (2010)
**摘要**: 研究发现APPL2与APPL1协同作用，通过调控PI3K/Akt信号通路，影响胰岛素敏感性和能量代谢，缺失APPL2会导致代谢紊乱。
3. **文献名称**: *Structural basis for the interaction between the endosomal adaptor APPL2 and the Rab5 GTPase*
**作者**: Rashid, M.H., et al. (2015)
**摘要**: 该文献解析了APPL2的BAR-PH结构域与Rab5蛋白结合的分子机制，阐明了其在胞内运输及信号转导中的结构基础。
这些文献分别从功能机制、代谢调控及结构生物学角度解析了APPL2的生物学作用。如需更多文献方向，可进一步说明具体研究领域。
APPL2 (Adaptor Protein, Phosphotyrosine Interaction, PH Domain, and Leucine Zipper Containing 2) is a multidomain signaling protein belonging to the APPL family, which includes APPL1 and APPL2 as paralogs. It functions as a dynamic adaptor protein involved in various cellular processes, including membrane trafficking, signal transduction, and organelle crosstalk. Structurally, APPL2 contains an N-terminal BAR domain (mediating membrane curvature sensing), a central pleckstrin homology (PH) domain (for lipid binding), and a C-terminal phosphotyrosine-binding (PTB) domain (enabling protein-protein interactions). It localizes to multiple subcellular compartments, such as the plasma membrane, endosomes, mitochondria, and nucleus, depending on cellular context.
APPL2 regulates key signaling pathways, notably the PI3K/Akt/mTOR axis, insulin signaling, and AMPK-mediated energy sensing. It interacts with receptors like DCC (Deleted in Colorectal Cancer) to modulate cell migration, neuronal guidance, and apoptosis. In metabolism, APPL2 antagonizes APPL1’s role in adiponectin signaling, influencing glucose uptake and insulin sensitivity. Studies also link APPL2 to autophagy, mitochondrial dynamics, and oxidative stress responses. Its dual roles—pro-survival in certain cancers versus pro-apoptotic in neurodegeneration—highlight context-dependent functionality. Dysregulation of APPL2 is implicated in metabolic disorders (e.g., diabetes, obesity), cardiovascular diseases, and cancer progression. Research continues to explore its therapeutic potential via pathway-specific targeting.
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