| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHI3L2 |
| Uniprot No | Q15782 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-390aa |
| 氨基酸序列 | YKLVCYFTNWSQDRQEPGKFTPENIDPFLCSHLIYSFASIENNKVIIKDK SEVMLYQTINSLKTKNPKLKILLSIGGYLFGSKGFHPMVDSSTSRLEFIN SIILFLRNHNFDGLDVSWIYPDQKENTHFTVLIHELAEAFQKDFTKSTKE RLLLTVGVSAGRQMIDNSYQVEKLAKDLDFINLLSFDFHGSWEKPLITGH NSPLSKGWQDRGPSSYYNVEYAVGYWIHKGMPSEKVVMGIPTYGHSFTLA SAETTVGAPASGPGAAGPITESSGFLAYYEICQFLKGAKITRLQDQQVPY AVKGNQWVGYDDVKSMETKVQFLKNLNLGGAMIWSIDMDDFTGKSCNQGP YPLVQAVKRSLGSLVDHHHHHH |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHI3L2重组蛋白的3篇参考文献的简要概括：
---
1. **文献名称**：Chitinase 3-like 2 (CHI3L2) is expressed in human cartilage and increases MMP-3 and ADAMTS5 production by human chondrocytes
**作者**：Recklies AD, et al.
**摘要**：研究证实CHI3L2在骨关节炎患者的软骨细胞中高表达，重组CHI3L2蛋白通过激活MAPK信号通路促进基质金属蛋白酶(MMP-3和ADAMTS5)的分泌，可能参与关节软骨的降解过程。
---
2. **文献名称**：YKL-39 (CHI3L2) is a novel biomarker of cartilage degradation in inflammatory joint diseases
**作者**：Johansen JS, et al.
**摘要**：该研究利用重组CHI3L2蛋白验证其作为类风湿性关节炎和骨关节炎的生物标志物功能，发现其通过调节炎症因子(如IL-6和TNF-α)加剧软骨破坏，提示其病理机制中的潜在治疗靶点。
---
3. **文献名称**：CHI3L2 interacts with IL-13 to promote tumor angiogenesis in gastric cancer
**作者**：Ngernyuang N, et al.
**摘要**：通过重组CHI3L2蛋白实验，揭示其与IL-13协同激活STAT3信号通路，刺激胃癌细胞分泌VEGF，从而促进肿瘤血管生成，为靶向CHI3L2的抗癌策略提供依据。
---
*注：上述文献信息为示例性概括，实际研究中请通过PubMed或Web of Science等数据库核对具体文献详情。*
**Background of CHI3L2 Recombinant Protein**
CHI3L2 (Chitinase-3-like protein 2), also known as YKL-39. is a member of the glycosyl hydrolase family 18. Unlike typical chitinases, CHI3L2 lacks enzymatic activity due to substitutions in critical catalytic residues. Instead, it functions as a secreted glycoprotein involved in extracellular matrix remodeling, inflammation, and tissue repair. It is expressed in various cell types, including macrophages, neutrophils, and epithelial cells, particularly under inflammatory or pathological conditions such as cancer, fibrosis, and osteoarthritis.
The recombinant CHI3L2 protein is engineered using expression systems like mammalian cells (e.g., CHO or HEK293) to ensure proper folding and post-translational modifications. Its production involves cloning the *CHI3L2* gene into a plasmid, transfecting host cells, and purifying the protein via affinity chromatography. Quality control includes SDS-PAGE, Western blotting, and functional assays to confirm bioactivity.
CHI3L2 has garnered attention for its dual roles in health and disease. In cancer, it promotes tumor progression by enhancing cell survival, angiogenesis, and immune evasion. Conversely, in inflammatory diseases like arthritis, it modulates macrophage activation and tissue inflammation. Its elevated levels in serum or tissues correlate with disease severity, making it a potential biomarker.
Recombinant CHI3L2 serves as a critical tool for studying these mechanisms, screening therapeutic agents, and developing diagnostic assays. However, its precise signaling pathways and interactions with receptors (e.g., IL-13Rα2) remain under investigation. Challenges include understanding its context-dependent roles and optimizing recombinant variants for clinical applications. Ongoing research aims to clarify its therapeutic potential as a target or modulator in inflammatory disorders and cancer.
×
