| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATG4D |
| Uniprot No | Q86TL0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-141aa |
| 氨基酸序列 | MGGKPRHSLYFIGYQDDFLLYLDPHYCQPTVDVSQADFPLESFHCTSPRKMAFAKMDPSCTVGFYAGDRKEFETLCSELTRVLSSSSATERYPMFTLAEGHAQDHSLDDLCSQLAQPTLRLPRTGRLLRAKRPSSEDFVFL |
| 分子量 | 42.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于ATG4D的相关文献及其摘要概括：
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1. **文献名称**: *Structural Basis for the Specificity and Catalysis of Human Atg4B Responsible for Mammalian Autophagy*
**作者**: Kumanomidou T, et al.
**摘要**: 本研究解析了人源ATG4B的晶体结构，并探讨了ATG4家族成员(包括ATG4D)在底物识别和催化机制中的差异。研究发现ATG4D对LC3/GABARAP家族蛋白的水解活性较低，可能与底物结合口袋结构差异有关。(研究方向：酶结构与底物特异性)
2. **文献名称**: *Regulation of Mammalian Autophagy by Class III Phosphatidylinositol 3-Kinase and Atg4 Proteases*
**作者**: Marino G, et al.
**摘要**: 文章系统性评估了ATG4蛋白酶在自噬体成熟中的作用，揭示ATG4D在特定生理条件下(如营养缺失)通过调控LC3的脂化状态影响自噬通量，且其功能部分冗余于其他同工酶(如ATG4B)。(研究方向：功能调控与信号通路)
3. **文献名称**: *Comprehensive Analysis of Human ATG4 Family Members as Novel Biomarkers in Pan-Cancer*
**作者**: Li X, et al.
**摘要**: 通过生物信息学分析，发现ATG4D在多种癌症中的表达异常及预后相关性，提示其可能参与肿瘤发生和化疗耐药机制，为靶向自噬的癌症治疗提供潜在靶点。(研究方向：疾病关联与转化医学)
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以上文献涉及ATG4D的结构功能、生理调控及疾病中的角色，部分研究需结合实验数据验证其机制。如需具体全文，可通过PubMed或期刊数据库检索标题获取。
Autophagy-related cysteine protease ATG4D (also known as AUTL1) is a member of the ATG4 protease family critical for autophagy, a conserved cellular degradation process. ATG4D specifically processes ATG8-family proteins (e.g., LC3. GABARAP) by cleaving their C-terminal residues to expose a glycine essential for their conjugation to phosphatidylethanolamine (PE) on autophagosome membranes. This lipidation step facilitates autophagosome expansion and cargo sequestration. Unlike other ATG4 isoforms (ATG4A-C), ATG4D exhibits lower basal activity but is selectively activated under stress, suggesting specialized regulatory roles. Studies implicate ATG4D in both canonical autophagy and non-autophagic pathways, including protein trafficking and apoptosis regulation. Recombinant ATG4D, produced via heterologous expression systems (e.g., E. coli, mammalian cells), enables structural and functional studies, such as substrate specificity profiling, enzyme kinetics, and inhibitor screening. Dysregulation of ATG4D is linked to cancers, neurodegenerative disorders, and infectious diseases, making it a potential therapeutic target. Recent research focuses on its post-translational modifications, interaction partners, and redundancy among ATG4 family members, aiming to delineate isoform-specific functions in cellular homeostasis and disease.
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