| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP5G1 |
| Uniprot No | P05496 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-136aa |
| 氨基酸序列 | TRGLIRPVSASFLSSPVNSSKQPSYSNFPLQVARREFQTSVVSRDIDTAAKFIGAGAATVGVAGSGAGIGTVFGSLIIGYARNPSLKQQLFSYAILGFALSEAMGLFCLMVAFLILFAM |
| 分子量 | 38.83 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ATP合成酶脂质结合蛋白(ATP5G1)的3篇参考文献及其摘要要点：
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**1. 文献名称**：**"ATP5G1 regulates mitochondrial ATP synthesis and cellular energy metabolism in cancer cells"**
**作者**：Smith A, et al. (2021)
**摘要**：研究发现ATP5G1通过调控线粒体ATP合酶复合物的组装，影响肿瘤细胞的能量代谢。敲低ATP5G1导致线粒体膜电位下降和细胞凋亡增加，表明其可能作为癌症治疗的潜在靶点。
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**2. 文献名称**：**"Structural insights into the lipid-binding properties of ATP5G1 in mitochondrial ATP synthase"**
**作者**：Li J, et al. (2019)
**摘要**：通过冷冻电镜技术解析ATP5G1的脂质结合结构域，揭示其与线粒体内膜磷脂的相互作用机制，阐明了其在质子转运和ATP合成中的关键作用。
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**3. 文献名称**：**"ATP5G1 overexpression promotes neurodegenerative disease via oxidative stress and mitochondrial dysfunction"**
**作者**：Wang Y, et al. (2020)
**摘要**：研究发现ATP5G1在阿尔茨海默病模型中表达上调，过量表达导致线粒体ROS水平升高、神经细胞死亡，提示其参与神经退行性病变的病理过程。
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(注：以上文献为虚拟示例，实际研究中建议通过PubMed或Web of Science检索具体文献。)
ATP synthase lipid-binding protein, also known as ATP5G1 or subunit c of mitochondrial ATP synthase, is a critical component of the F0 complex in ATP synthase (Complex V). This enzyme drives the final step of oxidative phosphorylation, generating ATP by utilizing the proton gradient across the inner mitochondrial membrane. ATP5G1. encoded by the ATP5G1 gene, is one of three isoforms (C1. C2. C3) of subunit c and plays a vital role in forming the proton-translocating channel within the F0 sector.
Structurally, ATP5G1 is a hydrophobic proteolipid that spans the membrane twice, contributing to the assembly and stability of the ATP synthase rotor ring. Its lipid-binding properties enable interactions with the mitochondrial membrane, facilitating proton translocation. Dysregulation of ATP5G1 has been linked to mitochondrial disorders, neurodegenerative diseases, and cancer, as altered ATP synthase activity disrupts cellular energy metabolism.
Recombinant ATP5G1 is widely used to study mitochondrial bioenergetics, structure-function relationships of ATP synthase, and pathogenic mechanisms in disease models. Research also explores its potential as a biomarker or therapeutic target for conditions associated with mitochondrial dysfunction.
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