| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP6V1A |
| Uniprot No | P38606 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-617aa |
| 氨基酸序列 | MDFSKLPKILDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEETSGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPRGVNVSALSRDIKWDFTPCKNLRVGSHITGGDIYGIVSENSLIKHKIMLPPRNRGTVTYIAPPGNYDTSDVVLELEFEGVKEKFTMVQVWPVRQVRPVTEKLPANHPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGDILYKLSSMKFKDPLKDGEAKIKSDYAQLLEDMQNAFRSLED |
| 分子量 | 93.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP6V1A的3篇代表性文献摘要(基于模拟虚构示例，实际文献需通过学术数据库核实)：
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1. **文献名称**：*Structural insights into the V-ATPase: ATP6V1A subunit function in proton translocation*
**作者**：Smith J, et al.
**摘要**：通过冷冻电镜解析ATP6V1A的晶体结构，揭示其在V型ATP酶复合体中的催化核心作用，阐明质子跨膜转运的分子机制。
2. **文献名称**：*ATP6V1A mutations cause autosomal recessive distal renal tubular acidosis*
**作者**：Karet FE, et al.
**摘要**：研究发现ATP6V1A基因突变导致肾小管酸中毒，表明该亚基在维持肾脏酸平衡中具有关键功能。
3. **文献名称**：*ATP6V1A promotes breast cancer metastasis via lysosomal secretion*
**作者**：Lee S, et al.
**摘要**：证实ATP6V1A通过调控溶酶体酸化促进乳腺癌细胞侵袭转移，为靶向V-ATP酶的癌症治疗提供依据。
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**建议**：实际文献检索可使用PubMed/Google Scholar，结合关键词“ATP6V1A”和“V-ATPase”，筛选结构、疾病或功能相关研究。
**Background of Recombinant Human V-Type Proton ATPase Catalytic Subunit A (ATP6V1A)**
The V-type proton ATPase (V-ATPase) is a multi-subunit enzyme critical for acidifying intracellular compartments, such as lysosomes and endosomes, by pumping protons across membranes via ATP hydrolysis. The ATP6V1A gene encodes the catalytic subunit A of the V1 domain, which is responsible for ATP binding and hydrolysis, providing energy for proton translocation. This subunit is evolutionarily conserved, highlighting its essential role in cellular homeostasis. Structurally, ATP6V1A interacts with other V1 and V0 domain subunits to assemble the functional holoenzyme, localized to organellar and plasma membranes in a cell type-specific manner.
ATP6V1A-mediated proton transport regulates pH-dependent processes, including protein degradation, vesicular trafficking, and ion homeostasis. Dysfunction of ATP6V1A is linked to human diseases: recessive mutations cause autosomal recessive cutis laxa type 2B (ARCL2B), characterized by tissue laxity and developmental defects, while altered expression is implicated in renal tubular acidosis, neurodevelopmental disorders, and cancer. In cancer, V-ATPase overexpression enhances tumor invasiveness by modulating the tumor microenvironment.
Recombinant ATP6V1A facilitates mechanistic studies of V-ATPase assembly, activity, and disease-related mutations. It serves as a tool for drug screening, targeting pH regulation pathways in pathologies like osteoporosis and metastatic cancers. Research on ATP6V1A continues to unveil its structural dynamics and therapeutic potential, underscoring its centrality in cellular physiology and disease.
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