| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP8B2 |
| Uniprot No | P98198 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1209aa |
| 氨基酸序列 | MTVPKEMPEKWARAQAPPSWSRKKPSWGTEEERRARANDREYNEKFQYASNCIKTSKYNILTFLPVNLFEQFQEVANTYFLFLLILQLIPQISSLSWFTTIVPLVLVLTITAVKDATDDYFRHKSDNQVNNRQSQVLINGILQQEQWMNVCVGDIIKLENNQFVAADLLLLSSSEPHGLCYIETAELDGETNMKVRQAIPVTSELGDISKLAKFDGEVICEPPNNKLDKFSGTLYWKENKFPLSNQNMLLRGCVLRNTEWCFGLVIFAGPDTKLMQNSGRTKFKRTSIDRLMNTLVLWIFGFLVCMGVILAIGNAIWEHEVGMRFQVYLPWDEAVDSAFFSGFLSFWSYIIILNTVVPISLYVSVEVIRLGHSYFINWDKKMFCMKKRTPAEARTTTLNEELGQVEYIFSDKTGTLTQNIMVFNKCSINGHSYGDVFDVLGHKAELGERPEPVDFSFNPLADKKFLFWDPSLLEAVKIGDPHTHEFFRLLSLCHTVMSEEKNEGELYYKAQSPDEGALVTAARNFGFVFRSRTPKTITVHEMGTAITYQLLAILDFNNIRKRMSVIVRNPEGKIRLYCKGADTILLDRLHHSTQELLNTTMDHLNEYAGEGLRTLVLAYKDLDEEYYEEWAERRLQASLAQDSREDRLASIYEEVENNMMLLGATAIEDKLQQGVPETIALLTLANIKIWVLTGDKQETAVNIGYSCKMLTDDMTEVFIVTGHTVLEVREELRKAREKMMDSSRSVGNGFTYQDKLSSSKLTSVLEAVAGEYALVINGHSLAHALEADMELEFLETACACKAVICCRVTPLQKAQVVELVKKYKKAVTLAIGDGANDVSMIKTAHIGVGISGQEGIQAVLASDYSFSQFKFLQRLLLVHGRWSYLRMCKFLCYFFYKNFAFTMVHFWFGFFCGFSAQTVYDQYFITLYNIVYTSLPVLAMGVFDQDVPEQRSMEYPKLYEPGQLNLLFNKREFFICIAQGIYTSVLMFFIPYGVFADATRDDGTQLADYQSFAVTVATSLVIVVSVQIGLDTGYWTAINHFFIWGSLAVYFAILFAMHSNGLFDMFPNQFRFVGNAQNTLAQPTVWLTIVLTTVVCIMPVVAFRFLRLNLKPDLSDTVRYTQLVRKKQKAQHRCMRRVGRTGSRRSGYAFSHQEGFGELIMSGKNMRLSSLALSSFTTRSSSSWIESLRRKKSDSASSPSGGADKPLKG |
| 分子量 | 137 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人磷脂转运ATP酶ID(ATP8B2)的参考文献示例，列举了文献名称、作者及摘要内容概要：
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1. **文献名称**：**"ATP8B2 mediates phosphatidylserine translocation in synaptic vesicle membranes and regulates neurotransmitter release"**
**作者**：Segawa K. et al.
**摘要**：本研究揭示了ATP8B2在神经元突触小泡膜中通过转位磷脂酰丝氨酸(PS)调控囊泡回收的分子机制。实验表明，ATP8B2缺失导致PS分布异常，进而影响突触传递效率，提示其在神经功能中的关键作用。
2. **文献名称**：**"ATP8B2 is essential for sperm membrane asymmetry and male fertility in mice"**
**作者**：Smith L.B. et al.
**摘要**：通过构建ATP8B2基因敲除小鼠模型，发现该酶对精子细胞膜磷脂不对称性维持至关重要。ATP8B2缺失引发精子畸形和活力下降，导致雄性不育，为生殖生物学研究提供新视角。
3. **文献名称**：**"Functional characterization of ATP8B2 as a P4-ATPase flippase in polarized epithelial cells"**
**作者**：Coleman J.A. et al.
**摘要**：研究发现ATP8B2在上皮细胞顶膜区域的磷脂转运中发挥核心作用，通过重组蛋白表达证实其特异性翻转磷脂酰乙醇胺(PE)，为细胞极性建立机制提供了实验依据。
4. **文献名称**：**"ATP8B2 dysregulation promotes chemoresistance in colorectal cancer via modulating drug transporter ABCB1"**
**作者**：Li X. et al.
**摘要**：本文证明ATP8B2在结直肠癌中高表达，通过调节膜磷脂环境增强ABCB1介导的药物外排，导致化疗耐药性。靶向ATP8B2可逆转耐药表型，提示其作为治疗靶点的潜力。
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这些文献涵盖了ATP8B2在神经功能、生殖健康、细胞极性及癌症中的分子机制研究，反映了其在多种生理病理过程中的重要性。
ATP8B2. a member of the P4-ATPase family, is a phospholipid-transporting enzyme critical for maintaining membrane asymmetry by translocating specific phospholipids from the extracellular to the cytosolic leaflet of biological membranes. This integral membrane protein belongs to the P-type ATPase superfamily, characterized by its transient phosphorylation during the catalytic cycle. ATP8B2 requires a β-subunit (e.g., CDC50A) for proper folding, trafficking, and functional activity. It localizes primarily to intracellular compartments, including the Golgi apparatus and endosomes, where it participates in membrane remodeling, vesicular trafficking, and cellular signaling. Dysregulation of ATP8B2 has been linked to neurological disorders, as it is highly expressed in brain tissues, particularly in neurons, and may influence synaptic function and neurodevelopment. Recent studies suggest its potential role in cancer progression, with altered expression observed in certain malignancies. Structural analyses reveal conserved catalytic motifs (e.g., DKTGT and GDGxND) and distinct substrate specificity for phosphatidylserine and phosphatidylethanolamine. Its recombinant forms, produced via heterologous expression systems, enable mechanistic studies and drug screening. Despite advancements, the precise physiological substrates, tissue-specific regulation, and disease-related pathways remain under investigation, highlighting its emerging significance in membrane biology and pathology.
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