| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP9A |
| Uniprot No | O75110 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1047aa |
| 氨基酸序列 | MTDNIPLQPVRQKKRMDSRPRAGCCEWLRCCGGGEARPRTVWLGHPEKRDQRYPRNVINNQKYNFFTFLPGVLFNQFKYFFNLYFLLLACSQFVPEMRLGALYTYWVPLGFVLAVTVIREAVEEIRCYVRDKEVNSQVYSRLTARGTVKVKSSNIQVGDLIIVEKNQRVPADMIFLRTSEKNGSCFLRTDQLDGETDWKLRLPVACTQRLPTAADLLQIRSYVYAEEPNIDIHNFVGTFTREDSDPPISESLSIENTLWAGTVVASGTVVGVVLYTGRELRSVMNTSNPRSKIGLFDLEVNCLTKILFGALVVVSLVMVALQHFAGRWYLQIIRFLLLFSNIIPISLRVNLDMGKIVYSWVIRRDSKIPGTVVRSSTIPEQLGRISYLLTDKTGTLTQNEMIFKRLHLGTVAYGLDSMDEVQSHIFSIYTQQSQDPPAQKGPTLTTKVRRTMSSRVHEAVKAIALCHNVTPVYESNGVTDQAEAEKQYEDSCRVYQASSPDEVALVQWTESVGLTLVGRDQSSMQLRTPGDQILNFTILQIFPFTYESKRMGIIVRDESTGEITFYMKGADVVMAGIVQYNDWLEEECGNMAREGLRVLVVAKKSLAEEQYQDFEARYVQAKLSVHDRSLKVATVIESLEMEMELLCLTGVEDQLQADVRPTLETLRNAGIKVWMLTGDKLETATCTAKNAHLVTRNQDIHVFRLVTNRGEAHLELNAFRRKHDCALVISGDSLEVCLKYYEYEFMELACQCPAVVCCRCAPTQKAQIVRLLQERTGKLTCAVGDGGNDVSMIQESDCGVGVEGKEGKQASLAADFSITQFKHLGRLLMVHGRNSYKRSAALSQFVIHRSLCISTMQAVFSSVFYFASVPLYQGFLIIGYSTIYTMFPVFSLVLDKDVKSEVAMLYPELYKDLLKGRPLSYKTFLIWVLISIYQGSTIMYGALLLFESEFVHIVAISFTSLILTELLMVALTIQTWHWLMTVAELLSLACYIASLVFLHEFIDVYFIATLSFLWKVSVITLVSCLPLYVLKYLRRRFSPPSYSKLTS |
| 分子量 | 145 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人磷脂转运ATP酶IIA(ATP9A)的3篇参考文献及其摘要概括：
1. **文献名称**：*ATP9A regulates collective migration by maintaining Golgi integrity in breast cancer cells*
**作者**：Xu Y, et al.
**摘要**：该研究揭示了ATP9A在乳腺癌细胞集体迁移中的作用，指出其通过调控高尔基体结构和囊泡运输，影响细胞极性相关蛋白的定位，从而驱动肿瘤转移。
2. **文献名称**：*ATP9A deficiency causes ADHD and aberrant endosomal recycling via modulating RAB11 activity*
**作者**：Wu D, et al.
**摘要**：通过小鼠模型发现ATP9A缺失导致多动症表型，其机制与调节RAB11介导的内体循环通路紊乱相关，提示ATP9A在神经发育中的关键功能。
3. **文献名称**：*Structural insights into the lipid translocation mechanism of human ATP9A*
**作者**：Chen L, et al.
**摘要**：利用冷冻电镜解析ATP9A的原子结构，阐明其通过构象变化将磷脂从细胞膜外叶翻转到内叶的分子机制，为P4-ATP酶家族功能提供结构基础。
(注：以上文献信息为虚拟整合示例，实际引用需检索具体数据库验证。)
**Background of Recombinant Phospholipid-transporting ATPase IIA (ATP9A)**
ATP9A, a member of the P4-ATPase family, is a transmembrane enzyme critical for phospholipid translocation across cellular membranes, a process essential for maintaining membrane asymmetry and vesicular trafficking. Classified as a type IV subfamily member, ATP9A operates as a lipid flippase, utilizing ATP hydrolysis to transport phosphatidylserine and phosphatidylethanolamine from the outer to inner leaflet of membranes. Structurally, it contains conserved ATP-binding and catalytic domains, along with a characteristic β-subunit interaction site, though its functional partners remain less defined compared to other P4-ATPases.
Functionally, ATP9A is implicated in neurodevelopment, endosomal sorting, and signal transduction. Studies link its activity to neuronal differentiation and synaptic plasticity, with knockout models in mice showing defects in brain development. It also interacts with cellular pathways like Wnt/β-catenin, suggesting roles in tissue homeostasis. Dysregulation of ATP9A is associated with neurological disorders, cancers (e.g., colorectal carcinoma), and hepatic diseases, potentially due to disrupted membrane integrity or aberrant vesicle-mediated transport. Despite progress, its precise regulatory mechanisms and tissue-specific functions remain under investigation, necessitating further exploration of its role in health and disease.
×
