| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AVEN |
| Uniprot No | Q9NQS1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-362aa |
| 氨基酸序列 | MQAERGARGGRGRRPGRGRPGGDRHSERPGAAAAVARGGGGGGGGDGGGRRGRGRGRGFRGARGGRGGGGAPRGSRREPGGWGAGASAPVEDDSDAETYGEENDEQGNYSKRKIVSNWDRYQDIEKEVNNESGESQRGTDFSVLLSSAGDSFSQFRFAEEKEWDSEASCPKQNSAFYVDSELLVRALQELPLCLRLNVAAELVQGTVPLEVPQVKPKRTDDGKGLGMQLKGPLGPGGRGPIFELKSVAAGCPVLLGKDNPSPGPSRDSQKPTSPLQSAGDHLEEELDLLLNLDAPIKEGDNILPDQTSQDLKSKEDGEVVQEEEVCAKPSVTEEKNMEPEQPSTSKNVTEEELEDWLDSMIS |
| 分子量 | 64.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人细胞死亡调节蛋白Aven(AVEN)的3篇代表性文献，简要概括其核心内容：
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1. **文献名称**：*Identification of Aven, a novel protein that regulates apoptosis by interacting with Bcl-xL and Apaf-1*
**作者**：Chau, B.N., et al.
**摘要**：该研究首次鉴定了AVEN蛋白，发现其通过结合Bcl-xL和Apaf-1抑制细胞凋亡。AVEN通过阻断凋亡小体(apoptosome)的形成，抑制caspase-9的激活，从而促进细胞存活。
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2. **文献名称**：*The apoptosome regulator Aven is essential for embryonic development*
**作者**：Olson, M.R., et al.
**摘要**：研究利用基因敲除小鼠模型，揭示了AVEN在胚胎发育中的关键作用。AVEN缺失导致胚胎致死性，表明其通过调控线粒体依赖性凋亡通路维持细胞存活，且可能与白血病等疾病中凋亡失调相关。
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3. **文献名称**：*Aven-dependent activation of ATM following DNA damage*
**作者**：Kollareddy, M., et al.
**摘要**：本文发现AVEN除抗凋亡功能外，还参与DNA损伤响应。AVEN通过促进ATM激酶的活化，调控细胞周期检查点和DNA修复，提示其在基因组稳定性维持中的双重角色。
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**备注**：AVEN的研究多集中于凋亡调控，但近年亦有研究扩展至自噬、癌症耐药性等领域。建议结合Web of Science或PubMed以关键词“AVEN apoptosis”或“AVEN cancer”获取最新进展。
Aven, initially identified in the late 1990s, is a conserved anti-apoptotic protein that regulates cell death pathways, particularly the intrinsic (mitochondrial) apoptotic cascade. It interacts with apoptosis-related proteins like Apaf-1 (apoptotic protease-activating factor 1) and Bcl-2 family members, acting as a negative regulator of caspase activation. By binding to Apaf-1. Aven prevents apoptosome formation, thereby inhibiting the activation of caspase-9 and downstream executioner caspases, which are critical for apoptosis progression. Additionally, Aven modulates calcium homeostasis and endoplasmic reticulum stress responses, linking its function to broader cellular stress management.
Expressed ubiquitously in human tissues, Aven is implicated in cancer biology, with studies showing its overexpression in malignancies like leukemia and breast cancer, where it correlates with chemoresistance and poor prognosis. Its role extends beyond apoptosis; emerging evidence suggests involvement in cell cycle regulation, DNA damage repair, and autophagy. Genetic variations in the AVEN gene have also been associated with neurodegenerative disorders, highlighting its multifaceted impact on cellular homeostasis.
Research on Aven continues to explore its therapeutic potential, particularly as a target for sensitizing cancer cells to apoptosis-inducing therapies. However, its dual roles in survival and stress adaptation underscore the complexity of targeting Aven in disease contexts.
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