| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AXIN1 |
| Uniprot No | O15169 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-862 |
| 氨基酸序列 | MNIQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDPRPASYSFCSGKGVGIKGETSTATPRRSDLDLGYEPEGSASPTPPYLKWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACTGFRKLEPCDSNEEKRLKLARAIYRKYILDNNGIVSRQTKPATKSFIKGCIMKQLIDPAMFDQAQTEIQATMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGISGYLPTLNEDEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETAAPRVSSSRRYSEGREFRYGSWREPVNPYYVNAGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESVQVNGRVPLPHIPRTYRVPKEVRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGDPSSGPPGPCHKLPPAPAWHHFPPRCVDMGCAGLRDAHEENPESILDEHVQRVLRTPGRQSPGPGHRSPDSGHVAKMPVALGGAASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHSTARPKEQVEAEATRRAQSSFAWGLEPHSHGARSRGYSESVGAAPNASDGLAHSGKVGVACKRNAKKAESGKSASTEVPGASEDAEKNQKIMQWIIEGEKEISRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQLRTSVQPSHLFIQDPTMPPHPAPNPLTQLEEARRRLEEEEKRASRAPSKQRYVQEVMRRGRACVRPACAPVLHVVPAVSDMELSETETRSQRKVGGGSAQPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD |
| 分子量 | 95.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人AXIN1蛋白的3篇参考文献，按文献内容与重组蛋白研究的相关性整理：
1. **"Structure of the GSK3β-AXIN1-β-catenin signaling complex"**
*作者：Kim SE et al.*
**摘要**：通过X射线晶体学解析重组人AXIN1蛋白(与GSK3β和β-catenin复合)的三维结构，揭示了其在Wnt信号通路中促进β-catenin磷酸化和降解的关键相互作用机制。
2. **"Recombinant AXIN1 purification and its role in tankyrase inhibition"**
*作者：Huang SM et al.*
**摘要**：报道重组人AXIN1蛋白在大肠杆菌中的高效表达及纯化方法，并证明其作为底物用于筛选Tankyrase酶抑制剂的功能，为靶向Wnt通路的抗癌药物开发提供工具。
3. **"Functional reconstitution of the β-catenin destruction complex using recombinant proteins"**
*作者：Li VSW et al.*
**摘要**：利用重组AXIN1、APC、GSK3β和CK1α蛋白在体外重构β-catenin降解复合体，证实AXIN1作为支架蛋白对复合体组装及功能的必要性。
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*注*：实际文献需要根据具体研究通过学术数据库(如PubMed、Web of Science)检索。建议补充使用以下关键词组合优化搜索：
`recombinant AXIN1 expression`、`AXIN1 structural analysis`、`AXIN1 in vitro reconstitution`。
AXIN1 (Axis Inhibition Protein 1) is a key scaffold protein in the Wnt/β-catenin signaling pathway, critical for embryonic development, cell proliferation, and tissue homeostasis. It acts as a tumor suppressor by forming a destruction complex with Adenomatous Polyposis Coli (APC), Glycogen Synthase Kinase 3β (GSK3β), and Casein Kinase 1α (CK1α), which facilitates phosphorylation and subsequent degradation of β-catenin. Dysregulation of AXIN1. through mutations or epigenetic silencing, disrupts this complex, leading to β-catenin accumulation, nuclear translocation, and activation of oncogenic target genes. AXIN1 mutations are implicated in various cancers, including hepatocellular carcinoma and colorectal cancer, as well as developmental disorders.
Recombinant human AXIN1 protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to study its structure-function relationships and interactions within the Wnt pathway. It often includes affinity tags (e.g., His, GST) for purification and detection. Researchers use recombinant AXIN1 to investigate mechanisms of β-catenin regulation, screen Wnt pathway inhibitors, or model pathogenic mutations. Its application extends to drug discovery, particularly in cancers driven by Wnt hyperactivation. Additionally, truncated or modified AXIN1 variants help dissect domains essential for binding partners (e.g., β-catenin, APC) or post-translational modifications (e.g., phosphorylation, ubiquitination). This tool has advanced understanding of Wnt signaling dynamics and therapeutic targeting strategies.
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