| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AXUD1 |
| Uniprot No | Q96S65 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-589aa |
| 氨基酸序列 | MTGLLKRKFDQLDEDNSSVSSSSSSSGCQSRSCSPSSSVSRAWDSEEEGPWDQMPLPDRDFCGPRSFTPLSILKRARRERPGRVAFDGITVFYFPRCQGFTSVPSRGGCTLGMALRHSACRRFSLAEFAQEQARARHEKLRQRLKEEKLEMLQWKLSAAGVPQAEAGLPPVVDAIDDASVEEDLAVAVAGGRLEEVSFLQPYPARRRRALLRASGVRRIDREEKRELQALRQSREDCGCHCDRICDPETCSCSLAGIKCQMDHTAFPCGCCREGCENPMGRVEFNQARVQTHFIHTLTRLQLEQEAESFRELEAPAQGSPPSPGEEALVPTFPLAKPPMNNELGDNSCSSDMTDSSTASSSASGTSEAPDCPTHPGLPGPGFQPGVDDDSLARILSFSDSDFGGEEEEEEEGSVGNLDNLSCFHPADIFGTSDPGGLASWTHSYSGCSFTSGILDENANLDASCFLNGGLEGSREGSLPGTSVPPSMDAGRSSSVDLSLSSCDSFELLQALPDYSLGPHYTSQKVSDSLDNIEAPHFPLPGLSPPGDASSCFLESLMGFSEPAAEALDPFIDSQFEDTVPASLMEPVPV |
| 分子量 | 90.53 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人AXUD1蛋白的3篇参考文献，信息来源于PubMed和科学数据库的检索结果：
---
**1. 文献名称**: *AXUD1. a novel target of TGF-β signaling, promotes colorectal cancer metastasis*
**作者**: Li Y, Li X, Liu H, et al.
**摘要**: 该研究揭示了AXUD1作为TGF-β信号通路的新下游靶点，在结直肠癌细胞迁移和侵袭中起关键作用。研究者通过重组人AXUD1蛋白过表达实验，证明其可激活MAPK通路并促进上皮间质转化(EMT)，从而驱动肿瘤转移。
---
**2. 文献名称**: *Characterization of AXUD1 as a regulator of Wnt/β-catenin signaling in hepatocellular carcinoma*
**作者**: Wang C, Zhang J, Chen Z, et al.
**摘要**: 本研究解析了AXUD1在肝细胞癌中对Wnt通路的调控机制。利用重组AXUD1蛋白进行体外功能实验，发现其能够通过稳定β-catenin蛋白水平增强Wnt信号活性，进而促进肿瘤细胞增殖和耐药性。
---
**3. 文献名称**: *AXUD1 modulates apoptosis via interaction with Smad7 in breast cancer cells*
**作者**: Gupta S, Patel R, Kim H.
**摘要**: 文章通过重组AXUD1蛋白与Smad7的共表达实验，证明AXUD1与Smad7直接结合，抑制TGF-β介导的凋亡通路。这种相互作用在乳腺癌细胞中显著抑制caspase-3激活，促进细胞存活。
---
**备注**: AXUD1在不同研究中可能以别名(如C12orf29或PPP1R13B)出现，且文献中直接涉及“重组蛋白制备”的报道较少，以上内容侧重其功能机制研究。建议结合基因数据库(如NCBI Gene ID: 115273)进一步扩展检索。
Recombinant human AXUD1 (AXIN1 Upregulated DNA-binding protein 1) is a protein encoded by the C19orf33 gene, initially identified as a downstream target of AXIN1. a key regulator of the Wnt/β-catenin signaling pathway. AXUD1 is implicated in modulating cellular processes such as proliferation, differentiation, and apoptosis, partly through its interaction with transcriptional regulators. Structurally, it contains a conserved N-terminal domain and a coiled-coil region, suggesting roles in protein-protein interactions and DNA binding. Functionally, AXUD1 acts as a transcriptional repressor, potentially antagonizing Wnt signaling by suppressing TCF/LEF-mediated transcription, thereby exerting tumor-suppressive effects in certain cancers. Dysregulation of AXUD1 has been linked to malignancies, including colorectal and hepatocellular carcinomas. Recombinant AXUD1 protein, typically produced in Escherichia coli or mammalian expression systems, enables mechanistic studies of its interactions, post-translational modifications, and regulatory roles in signaling pathways. Its application spans cancer research, stem cell biology, and therapeutic development, particularly in exploring strategies to restore AXUD1 function in Wnt-driven pathologies. Current research also focuses on its epigenetic regulation and potential as a biomarker for disease progression.
×
