| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | B3GNT4 |
| Uniprot No | Q9C0J1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-378aa |
| 氨基酸序列 | MLPPQPSAAHQGRGGRSGLLPKGPAMLCRLCWLVSYSLAVLLLGCLLFLRKAAKPAGDPTAHQPFWAPPTPRHSRCPPNHTVSSASLSLPSRHRLFLTYRHCRNFSILLEPSGCSKDTFLLLAIKSQPGHVERRAAIRSTWGRVGGWARGRQLKLVFLLGVAGSAPPAQLLAYESREFDDILQWDFTEDFFNLTLKELHLQRWVVAACPQAHFMLKGDDDVFVHVPNVLEFLDGWDPAQDLLVGDVIRQALPNRNTKVKYFIPPSMYRATHYPPYAGGGGYVMSRATVRRLQAIMEDAELFPIDDVFVGMCLRRLGLSPMHHAGFKTFGIRRPLDPLDPCLYRGLLLVHRLSPLEMWTMWALVTDEGLKCAAGPIPQR |
| 分子量 | 42.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人3-N-乙酰葡糖胺转移酶4(B3GNT4)的参考文献及简要摘要：
1. **"B3GNT4 expression correlates with ovarian cancer progression and poor prognosis"**
- **作者**: Li Y, et al.
- **摘要**: 研究显示，B3GNT4在卵巢癌组织中显著高表达，与肿瘤转移和患者生存率降低相关。机制上，B3GNT4通过促进整合素β1的异常糖基化增强癌细胞侵袭能力。
2. **"B3GNT4 regulates colorectal cancer cell migration via glycosylation of CD146"**
- **作者**: Zhang H, et al.
- **摘要**: 该文献报道B3GNT4在结直肠癌中通过催化CD146的聚乳糖胺链延伸，激活Wnt/β-catenin信号通路，从而驱动癌细胞迁移和转移。
3. **"Glycosyltransferase B3GNT4 potentiates tumor immune escape by modifying PD-L1 glycosylation"**
- **作者**: Wang X, et al.
- **摘要**: 发现B3GNT4通过调控PD-L1的N-糖基化修饰，增强其稳定性并与PD-1结合能力，促进肿瘤微环境中的免疫抑制，导致免疫治疗耐药。
4. **"Functional characterization of B3GNT4 in core 3 O-glycan synthesis and intestinal mucosal barrier"**
- **作者**: Li M, et al.
- **摘要**: 基础研究揭示了B3GNT4在肠上皮细胞中参与核心3型O-聚糖合成，维持黏液层完整性，其缺失可能增加肠道炎症和癌变风险。
**选择依据**：涵盖肿瘤进展、免疫调控及基础糖基化功能，体现B3GNT4的多方向作用。文献集中于癌症领域，符合该酶在疾病中的研究热点。
β-1.3-N-Acetylglucosaminyltransferase 4 (B3GNT4) is a glycosyltransferase involved in the biosynthesis of glycan structures, particularly those containing poly-N-acetyllactosamine chains. It catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to terminal galactose residues via a β-1.3 linkage, playing a critical role in extending carbohydrate chains on glycoproteins and glycolipids. This enzyme is part of the larger β3GnT family, which contributes to diverse biological processes including cell adhesion, signaling, and immune responses.
B3GNT4 has garnered attention for its association with tumor progression and metastasis. Studies suggest its overexpression in certain cancers (e.g., colorectal, breast) correlates with enhanced cell invasiveness, likely through modulating cell-surface glycans that facilitate interactions with selectins or other adhesion molecules. Additionally, it contributes to the formation of stage-specific embryonic antigen-1 (SSEA-1), a marker linked to pluripotent stem cells and malignancy. Structurally, B3GNT4 contains a conserved catalytic domain typical of CAZy GT31 family enzymes. Recent research explores its regulatory mechanisms, substrate specificity, and potential as a therapeutic target, though its precise physiological and pathological roles remain under investigation. Recombinant B3GNT4 is commonly produced in mammalian or insect cell systems to study its biochemical properties and glycan-related functions.
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