| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | B3GALT3 |
| Uniprot No | O75752 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-331aa |
| 氨基酸序列 | MASALWTVLPSRMSLRSLKWSLLLLSLLSFFVMWYLSLPHYNVIERVNWMYFYEYEPIYRQDFHFTLREHSNCSHQNLFLVILVTSHPSDVKARQAIRVTWGEKKSWWGYEVLTFFLLGQEAEKEDKMLALSLEDEHLLYGDIIRQDFLDTYNNLTLKTIMAFRWVTEFCPNAKYVMKTDTDVFINTGNLVKYLLNLNHSEKFFTGYPLIDNYSYRGFYQKTHISYQEYPFKVFPPYCSGLGYIMSRDLVPRIYEMMGHVKPIKFEDVYVGICLNLLKVNIHIPEDTNLFFLYRIHLDVCQLRRVIAAHGFSSKEIITFWQVMLRNTTCHY |
| 分子量 | 65.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人B3GALT3蛋白的研究文献简述：
1. **"Structural and functional characterization of human β3-galactosyltransferase 3 (B3GALT3)"**
- **作者**：Wang X et al.
- **摘要**：报道了重组人B3GALT3蛋白的体外表达及酶活性分析，揭示其对糖蛋白生物合成中多乳糖胺延伸的催化机制，并通过结构预测提出其底物结合位点特征。
2. **"B3GALT3 modulates tumor progression by regulating integrin-mediated signaling in colorectal cancer"**
- **作者**：Kim JH et al.
- **摘要**：通过重组蛋白和基因编辑技术，证明B3GALT3通过修饰整合素β1的糖基化影响肿瘤细胞迁移和侵袭，为结直肠癌治疗提供潜在靶点。
3. **"A systematic study of β3GalT3 substrate specificity using synthetic glycopeptide arrays"**
- **作者**：Schwarz F et al.
- **摘要**：利用重组B3GALT3蛋白分析其底物偏好性，发现其对含N-乙酰葡萄糖胺的O-连接糖链具有高亲和力，揭示该酶在黏蛋白糖基化中的关键作用。
注：以上文献标题及内容为模拟生成的科研主题示例，具体研究需通过PubMed/Google Scholar检索真实文献。建议结合关键词(B3GALT3、重组蛋白、糖基转移酶)筛选近五年高被引论文。
B3GALT3. also known as β-1.3-galactosyltransferase 3. is a key enzyme in glycoprotein and glycolipid biosynthesis. It catalyzes the transfer of galactose to terminal N-acetylglucosamine residues via a β-1.3 linkage, contributing to the formation of poly-N-acetyllactosamine chains and other glycoconjugates. This enzyme belongs to the β3GalT family and is expressed in various tissues, including placenta, brain, and kidney.
Recombinant human B3GALT3 protein is typically produced using engineered expression systems (e.g., mammalian cells, E. coli) to study its biochemical functions, substrate specificity, and interactions. Structurally, it contains conserved motifs of glycosyltransferases, such as a C-terminal catalytic domain and a DXD motif critical for metal ion coordination.
B3GALT3 plays roles in cellular adhesion, signaling, and immune responses, with dysregulation linked to diseases like cancer and congenital disorders of glycosylation (CDG). Its recombinant form aids in exploring therapeutic targets and molecular mechanisms underlying glycosylation-related pathologies. Current research focuses on its involvement in tumor progression, stem cell differentiation, and neuronal development.
×
