关键信息
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基因名
rnhA
- 应用
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别名
DHX9;DDX9;LKP;NDH2;ATP-dependent RNA helicase A
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9HSF6
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表达区间
1-199aa
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氨基酸序列
MPVVECDIQTARAALADAGASFSDGNSEHELWHADLGDAHAVAYADKLVVQGGSPTDITAVVQPDRGGRVHAYFDGASRGNPGPAAVGWVLVSGDGGIVAEGGDTIGRATNNQAEYDALIAALEAAADFGFDDIELRGDSQLVEKQLTGAWDTNDPDLRRKRVRARELLTGFDDWSITHVPRATNERADALANEALDDA
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The research on recombinant protein rnhA, which encodes RNase HI, has gained significant attention due to its essential role in various biological processes, particularly in RNA metabolism and DNA replication. RNase HI is an enzyme that specifically degrades the RNA strand of RNA-DNA hybrids, which is crucial for the removal of RNA primers during DNA replication, thereby ensuring genomic stability. Mutations or deficiencies in the rnhA gene can lead to a variety of cellular dysfunctions and diseases, making it a target for biomedical research. The production of recombinant rnhA allows for detailed studies of its enzymatic mechanisms, structural properties, and interactions with other cellular components. Moreover, understanding rnhA could have implications in developing therapeutic strategies against bacterial infections, as RNase HI is a potential drug target for antimicrobial agents. The advent of advanced expression systems and purification techniques has facilitated the efficient production and characterization of rnhA, enabling researchers to explore its functional roles and contributions to cellular homeostasis more comprehensively. This research not only enhances our understanding of fundamental cellular processes but also opens new avenues for biotechnology applications.
