| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIAE |
| Uniprot No | Q9HAT2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-523aa |
| 氨基酸序列 | MVAPGLVLGLVLPLILWADRSAGIGFRFASYINNDMVLQKEPAGAVIWGF GTPGATVTVTLRQGQETIMKKVTSVKAHSDTWMVVLDPMKPGGPFEVMAQ QTLEKINFTLRVHDVLFGDVWLCSGQSNMQMTVLQIFNATRELSNTAAYQ SVRILSVSPIQAEQELEDLVAVDLQWSKPTSENLGHGYFKYMSAVCWLFG RHLYDTLQYPIGLIASSWGGTPIEAWSSGRSLKACGVPKQGSIPYDSVTG PSKHSVLWNAMIHPLCNMTLKGVVWYQGESNINYNTDLYNCTFPALIEDW RETFHRGSQGQTERFFPFGLVQLSSDLSKKSSDDGFPQIRWHQTADFGYV PNPKMPNTFMAVAMDLCDRDSPFGSIHPRDKQTVAYRLHLGARALAYGEK NLTFEGPLPEKIELLAHKGLLNLTYYQQIQVQKKDNKIFEISCCSDHRCK WLPASMNTVSTQSLTLAIDSCHGTVVALRYAWTTWPCEYKQCPLYHPSSA LPAPPFIAFITDQGPGHQSNVAK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SIAE重组蛋白的3篇参考文献示例(注：文献信息为模拟内容，实际引用时请核实真实来源)：
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1. **文献名称**：*Autoimmune-associated Sialic Acid Acetylesterase (SIAE) Dysfunction Requires Catalytically Active Recombinant Protein for Functional Rescue*
**作者**：Surolia, T. et al.
**摘要**：研究通过重组表达人类SIAE蛋白，证实其酶活性对B细胞免疫耐受的调控作用，发现自身免疫患者中SIAE突变体的功能缺陷可通过野生型重组蛋白恢复，为治疗提供潜在靶点。
2. **文献名称**：*Structural Insights into Human Sialic Acid Acetylesterase by Recombinant Expression and X-ray Crystallography*
**作者**：Chavan, S. & Varki, A.
**摘要**：报道了利用大肠杆菌系统表达并纯化功能性SIAE重组蛋白，通过X射线晶体学解析其三维结构，揭示其催化活性中心的关键氨基酸残基，为酶机制研究奠定基础。
3. **文献名称**：*Recombinant SIAE Attenuates Rheumatoid Arthritis in Murine Models via Modulating Sialylation Patterns*
**作者**：Wang, L. et al.
**摘要**：在小鼠类风湿性关节炎模型中，外源性重组SIAE蛋白通过调控细胞表面唾液酸修饰，抑制炎症因子释放，显著缓解关节病理症状，提示其作为生物治疗剂的潜力。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“SIAE recombinant protein”或“sialic acid acetylesterase recombinant”获取近期研究。
Sialic acid acetylesterase (SIAE) is an enzyme encoded by the *SIAE* gene in humans, primarily involved in the modulation of sialic acid residues on glycoproteins and glycolipids. By hydrolyzing acetyl groups from 9-O-acetylated sialic acids, SIAE regulates cell-surface sialylation, a critical post-translational modification influencing cell-cell interactions, immune signaling, and pathogen recognition. Discovered in the early 2000s, SIAE gained attention for its role in B-cell tolerance. Studies showed that SIAE deficiency or mutations disrupt sialic acid homeostasis, leading to aberrant B-cell receptor signaling and autoimmune susceptibility, linking it to conditions like lupus and rheumatoid arthritis.
Recombinant SIAE protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional enzyme for research and therapeutic applications. Its structure typically includes a catalytic serine esterase domain and a lectin-like domain, though variants may lack transmembrane regions for solubility. Recombinant SIAE enables mechanistic studies of sialic acid metabolism, immune regulation, and disease pathways. It also serves as a tool for high-throughput screening of modulators targeting autoimmune disorders or cancer, where altered sialylation is prevalent. Recent advances in protein engineering have improved its stability and activity, facilitating structural studies (e.g., crystallography) to elucidate substrate-binding mechanisms. Despite progress, challenges remain in understanding its tissue-specific roles and therapeutic potential, driving ongoing research into SIAE's biology and applications.
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