| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MSR1 |
| Uniprot No | P21757 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 77-451aa |
| 氨基酸序列 | ADPKWETKNCSVSSTNANDITQSLTGKGNDSEEEMRFQEVFMEHMSNMEK RIQHILDMEANLMDTEHFQNFSMTTDQRFNDILLQLSTLFSSVQGHGNAI DEISKSLISLNTTLLDLQLNIENLNGKIQENTFKQQEEISKLEERVYNVS AEIMAMKEEQVHLEQEIKGEVKVLNNITNDLRLKDWEHSQTLRNITLIQG PPGPPGEKGDRGPTGESGPRGFPGPIGPPGLKGDRGAIGFPGSRGLPGYA GRPGNSGPKGQKGEKGSGNTLTPFTKVRLVGGSGPHEGRVEILHSGQWGT ICDDRWEVRVGQVVCRSLGYPGVQAVHKAAHFGQGTGPIWLNEVFCFGRE SSIEECKIRQWGTRACSHSEDAGVTCTLHHHHHH |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MSR1重组蛋白的示例参考文献(内容为模拟概括，建议通过学术数据库核实具体文献)：
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1. **"Recombinant MSR1 expression and ligand binding characterization in macrophage-derived foam cells"**
*Authors: Kodama T, Freeman M, et al.*
摘要：研究通过重组技术表达了MSR1蛋白，并验证其与氧化低密度脂蛋白(oxLDL)的结合能力，揭示了其在动脉粥样硬化中促进巨噬细胞转化为泡沫细胞的分子机制。
2. **"Structural insights into the macrophage scavenger receptor MSR1 via recombinant protein crystallization"**
*Authors: Suzuki H, Emi M, et al.*
摘要：利用重组MSR1蛋白进行X射线晶体学分析，解析其三维结构，阐明了其胶原蛋白结构域在配体识别中的关键作用，为靶向药物设计提供基础。
3. **"Recombinant MSR1 mediates bacterial clearance in innate immunity"**
*Authors: Peiser L, Gordon S, et al.*
摘要：通过体外实验证明重组MSR1蛋白能够结合革兰氏阳性菌表面成分，表明其在宿主防御中作为模式识别受体的功能，并可能参与炎症调控。
4. **"MSR1 recombinant protein as a potential therapeutic target in Alzheimer’s disease models"**
*Authors: Miki H, Yamamoto K, et al.*
摘要：研究重组MSR1蛋白在β-淀粉样蛋白(Aβ)清除中的作用，发现其过表达可减少神经细胞毒性，提示其作为阿尔茨海默病治疗靶点的潜力。
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**注意**：以上文献信息为示例性质，实际研究中请通过PubMed、Web of Science等平台检索关键词(如“MSR1 recombinant protein”“scavenger receptor expression”)获取准确文献。
**Background of MSR1 Recombinant Protein**
Macrophage scavenger receptor 1 (MSR1), also known as SR-A1 or CD204. is a class A scavenger receptor primarily expressed on macrophages and dendritic cells. It plays a critical role in innate immunity by mediating the recognition and clearance of modified lipoproteins, bacterial pathogens, and cellular debris. First identified in the 1990s, MSR1 is characterized by its ability to bind a broad range of ligands, including oxidized low-density lipoprotein (oxLDL), lipoteichoic acid, and β-amyloid fibrils, making it a key player in chronic inflammatory diseases, atherosclerosis, and neurodegenerative disorders.
Structurally, MSR1 contains a cytoplasmic N-terminal domain, a transmembrane region, and an extracellular domain featuring a collagen-like triple helix and a cysteine-rich C-terminal scavenger receptor cysteine-rich (SRCR) domain. Its trimeric configuration enables multivalent ligand interactions, facilitating pathogen phagocytosis and apoptotic cell removal.
Recombinant MSR1 protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional forms of the receptor for research and therapeutic applications. This recombinant approach allows for controlled studies of MSR1-ligand interactions, signaling pathways, and its role in disease mechanisms. For example, MSR1 has been implicated in tumor-associated macrophage (TAM) activity, influencing tumor progression and immune evasion, making it a potential target for cancer immunotherapy.
Additionally, recombinant MSR1 aids in developing inhibitors or modulators to treat conditions like atherosclerosis, Alzheimer’s disease, and chronic infections. Its study also sheds light on macrophage polarization and foam cell formation in vascular plaques. Despite its physiological importance, MSR1’s dual role in promoting inflammation and tissue repair underscores the complexity of targeting it therapeutically. Overall, MSR1 recombinant protein serves as a vital tool for unraveling its multifaceted biology and translational potential.
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