| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMOC1 |
| Uniprot No | Q9H4F8-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-435aa |
| 氨基酸序列 | MLPARCARLL TPHLLLVLVQ LSPARGHRTT GPRFLISDRD PQCNLHCSRT QPKPICASDG RSYESMCEYQ RAKCRDPTLG VVHRGRCKDA GQSKCRLERA QALEQAKKPQ EAVFVPECGE DGSFTQVQCH TYTGYCWCVT PDGKPISGSS VQNKTPVCSG SVTDKPLSQG NSGRKDDGSK PTPTMETQPV FDGDEITAPT LWIKHLVIKD SKLNNTNIRN SEKVYSCDQE RQSALEEAQQ NPREGIVIPE CAPGGLYKPV QCHQSTGYCW CVLVDTGRPL PGTSTRYVMP SCESDARAKT TEADDPFKDR ELPGCPEGKK MEFITSLLDA LTTDMVQAIN SAAPTGGGRF SEPDPSHTLE ERVVHWYFSQ LDSNSSNDIN KREMKPFKRY VKKKAKPKKC ARRFTDYCDL NKDKVISLPE LKGCLGVSKE VGRLV |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SMOC1重组蛋白的3篇代表性文献的简要概括(基于模拟生成，非真实文献)：
1. **标题**：*SMOC1 inhibits angiogenesis by selectively binding to extracellular matrix components*
**作者**：Smith A, et al.
**摘要**：研究利用重组SMOC1蛋白，发现其通过结合胶原蛋白和纤维连接蛋白抑制血管内皮细胞迁移，提示SMOC1可能通过调控细胞外基质抑制肿瘤血管生成。
2. **标题**：*Recombinant SMOC1 promotes osteogenic differentiation via BMP signaling activation*
**作者**：Zhang Y, et al.
**摘要**：通过大肠杆菌表达系统获得高纯度重组SMOC1蛋白，体外实验证实其通过激活BMP/Smad通路显著增强间充质干细胞的成骨分化，为骨修复提供潜在治疗策略。
3. **标题**：*Structural characterization of SMOC1 calcium-binding domains using recombinant protein*
**作者**：Tanaka K, et al.
**摘要**：利用昆虫细胞表达系统制备重组SMOC1蛋白，通过X射线晶体学解析其钙离子结合结构域的三维结构，揭示其与胚胎发育相关的分子作用机制。
注：以上文献信息为模拟生成，实际研究中建议通过PubMed或Web of Science以关键词"SMOC1 recombinant"检索最新文献。真实研究多集中于SMOC1在眼科畸形、肿瘤微环境调控及骨代谢疾病中的作用机制。
**Background of SMOC1 Recombinant Protein**
SMOC1 (Secreted Modular Calcium-Binding Protein 1) is a member of the SPARC (Secreted Protein Acidic and Rich in Cysteine) family, known for its role in extracellular matrix (ECM) organization and cellular interactions. It is a multidomain glycoprotein characterized by two calcium-binding EF-hand domains, a follistatin-like domain, and a thrombin-sensitive region. These structural features enable SMOC1 to interact with various ECM components, growth factors, and cell surface receptors, modulating signaling pathways critical for tissue development, repair, and homeostasis.
SMOC1 is expressed in multiple tissues, including the eye, kidney, and skeletal system, and is implicated in embryogenesis, angiogenesis, and wound healing. Studies highlight its regulatory role in bone morphogenetic protein (BMP) and Wnt signaling pathways, influencing cell proliferation, differentiation, and apoptosis. Dysregulation of SMOC1 is associated with developmental disorders, such as oculodentodigital dysplasia (ODDD), and pathological conditions like fibrosis and cancer metastasis.
Recombinant SMOC1 protein is engineered using expression systems (e.g., mammalian cells, *E. coli*) to produce purified, bioactive forms for research and therapeutic applications. It serves as a vital tool to study SMOC1's molecular mechanisms, screen drug candidates, or explore its potential in regenerative medicine. Recent advances in structural analysis and functional assays have expanded insights into its calcium-dependent interactions and tissue-specific roles.
Current research focuses on leveraging SMOC1 recombinant protein to develop targeted therapies for ECM-related diseases, enhance tissue engineering strategies, or elucidate its dual role as a tumor suppressor or promoter in different cancers. Its versatility in modulating cellular microenvironments positions SMOC1 as a promising candidate for biomedical innovation.
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