| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C18orf21 |
| Uniprot No | Q32NC0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-132aa |
| 氨基酸序列 | MVKKFKDSKSVLLITCKTCNRTVKHHGKSRSFVSTLKSNPATPASKLSLKTPERRTANPNHDMSGSKGKSPASVFRTPTSGQSVSTCSSKNTSKTKKHFSQLKMLLSQNESQKIPKVDFRNFLSSLKGGLLK |
| 分子量 | 40.92 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C18orf21蛋白的3篇代表性文献摘要概览(文献标题及内容为模拟示例，实际研究中可能需要结合具体数据库验证)：
1. **文献名称**: "C18orf21 encodes a novel protein involved in cellular stress response"
**作者**: Smith A, et al.
**摘要**: 通过体外重组表达C18orf21蛋白，发现其定位于线粒体，并在氧化应激条件下显著上调，可能通过调节线粒体膜电位参与细胞凋亡调控。
2. **文献名称**: "Functional characterization of recombinant human C18orf21 in DNA damage repair"
**作者**: Li X, et al.
**摘要**: 利用HEK293细胞表达重组C18orf21蛋白，证实其与BRCA1相互作用，并通过同源重组修复通路参与DNA双链断裂修复，提示其潜在肿瘤抑制功能。
3. **文献名称**: "Structural insights into the disordered regions of C18orf21 protein"
**作者**: Tanaka K, et al.
**摘要**: 采用NMR技术解析重组C18orf21蛋白的结构，揭示其C端无序区域在结合核转运蛋白Importin-α中的作用，为研究其亚细胞定位机制提供依据。
**注**：实际文献需通过PubMed、Web of Science等平台以“C18orf21”或“chromosome 18 open reading frame 21”为关键词检索。部分研究可能以该蛋白的其他命名(如PDF等)发表，需结合上下文确认。
**Background of Recombinant Human C18orf21 Protein**
The *C18orf21* gene, located on chromosome 18 (18q21.1), encodes a poorly characterized protein primarily studied for its potential roles in cellular processes and disease associations. Structurally, C18orf21 is predicted to contain a conserved domain of unknown function (DUF4552), suggesting possible involvement in molecular interactions or enzymatic activities, though its precise mechanism remains elusive. Recombinant human C18orf21 protein, produced via heterologous expression systems, enables functional studies to address knowledge gaps.
Current research links C18orf21 to cancer biology. Downregulated expression has been observed in gastric, colorectal, and hepatocellular carcinomas, implying tumor-suppressive potential. *In vitro* studies suggest it may inhibit cell proliferation, migration, and epithelial-mesenchymal transition (EMT) by modulating pathways like TGF-β/SMAD or Wnt/β-catenin. Additionally, C18orf21 has been implicated in neurological disorders, with genetic variants associated with epilepsy and intellectual disability. Its neuronal expression hints at roles in synaptic function or neurodevelopment.
Despite advances, functional validation is limited. Structural predictions using AlphaFold indicate a globular conformation with disordered regions, complicating mechanistic insights. Further studies are needed to clarify its molecular partners, signaling roles, and therapeutic relevance. Recombinant C18orf21 serves as a critical tool for unraveling its biological significance in health and disease.
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