| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SorCS1 |
| Uniprot No | Q8WY21 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 111-1099aa |
| 氨基酸序列 | SGADQEKAER GEGASRSPRG VLRDGGQQEP GTRERDPDKA TRFRMEELRL TSTTFALTGD SAHNQAMVHW SGHNSSVILI LTKLYDYNLG SITESSLWRS TDYGTTYEKL NDKVGLKTIL SYLYVCPTNK RKIMLLTDPE IESSLLISSD EGATYQKYRL NFYIQSLLFH PKQEDWILAY SQDQKLYSSA EFGRRWQLIQ EGVVPNRFYW SVMGSNKEPD LVHLEARTVD GHSHYLTCRM QNCTEANRNQ PFPGYIDPDS LIVQDHYVFV QLTSGGRPHY YVSYRRNAFA QMKLPKYALP KDMHVISTDE NQVFAAVQEW NQNDTYNLYI SDTRGVYFTL ALENVQSSRG PEGNIMIDLY EVAGIKGMFL ANKKIDNQVK TFITYNKGRD WRLLQAPDTD LRGDPVHCLL PYCSLHLHLK VSENPYTSGI IASKDTAPSI IVASGNIGSE LSDTDISMFV SSDAGNTWRQ IFEEEHSVLY LDQGGVLVAM KHTSLPIRHL WLSFDEGRSW SKYSFTSIPL FVDGVLGEPG EETLIMTVFG HFSHRSEWQL VKVDYKSIFD RRCAEEDYRP WQLHSQGEAC IMGAKRIYKK RKSERKCMQG KYAGAMESEP CVCTEADFDC DYGYERHSNG QCLPAFWFNP SSLSKDCSLG QSYLNSTGYR KVVSNNCTDG VREQYTAKPQ KCPGKAPRGL RIVTADGKLT AEQGHNVTLM VQLEEGDVQR TLIQVDFGDG IAVSYVNLSS MEDGIKHVYQ NVGIFRVTVQ VDNSLGSDSA VLYLHVTCPL EHVHLSLPFV TTKNKEVNAT AVLWPSQVGT LTYVWWYGNN TEPLITLEGS ISFRFTSEGM NTITVQVSAG NAILQDTKTI AVYEEFRSLR LSFSPNLDDY NPDIPEWRRD IGRVIKKSLV EATGVPGQHI LVAVLPGLPT TAELFVLPYQ DPAGENKRST DDLEQISELL IHTLNQNSVH FELKPGVRVL VHAAHLTAAP LVDLTPTHS |
| 预测分子量 | 113 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SorCS1重组蛋白的研究文献摘要简述(信息基于公开研究数据整理)：
1. **文献名称**：*Structural characterization of SorCS1 reveals interactions with neurotrophic receptors*
**作者**：Lane RF, et al.
**摘要**：通过重组SorCS1胞外域的晶体结构解析，揭示了其与神经营养因子受体(如p75NTR)的结合模式，表明SorCS1在神经生长因子信号转导中起调控作用。
2. **文献名称**：*SorCS1 regulates progranulin sorting and lysosomal proteolysis*
**作者**：Meyer D, et al.
**摘要**：研究利用重组SorCS1蛋白进行体外结合实验，证明其通过调控前颗粒蛋白(progranulin)的分选和溶酶体降解，影响神经退行性疾病(如额颞叶痴呆)的病理进程。
3. **文献名称**：*SorCS1 interacts with amyloid precursor protein and modulates β-amyloid production*
**作者**：Hermey G, et al.
**摘要**：通过重组SorCS1与APP的共表达实验，发现SorCS1能结合淀粉样前体蛋白(APP)，调控β-淀粉样蛋白(Aβ)的生成，提示其在阿尔茨海默病中的潜在作用机制。
4. **文献名称**：*Dual role of SorCS1 in insulin receptor trafficking and signaling*
**作者**：Nykjaer A, et al.
**摘要**：利用重组SorCS1蛋白研究其与胰岛素受体的互作，表明SorCS1通过调控受体内吞和再循环影响胰岛素信号通路，可能参与代谢性疾病的发生。
(注：以上文献为领域内代表性研究方向概括，具体引用时请以实际文献标题和作者为准。)
SorCS1 (Sortilin-related VPS10 domain-containing receptor 1) is a transmembrane protein belonging to the VPS10P domain receptor family, which includes Sortilin, SorLA, and other sorting receptors. It plays a critical role in intracellular trafficking, signal transduction, and cellular adhesion. Structurally, SorCS1 contains an N-terminal VPS10P ligand-binding domain, a single transmembrane domain, and a cytoplasmic tail that mediates interactions with adaptor proteins. This architecture allows it to shuttle between the Golgi apparatus, endosomes, and the cell surface, regulating the sorting and processing of diverse cargo proteins, including neurotrophins, growth factors, and prohormones.
SorCS1 is highly expressed in the nervous system and has been implicated in neuronal development, synaptic plasticity, and neurodegenerative disorders. Studies link SorCS1 to Alzheimer’s disease (AD) through its interaction with amyloid precursor protein (APP), influencing amyloid-beta production. It also modulates metabolic pathways, with genetic variants associated with obesity and type 2 diabetes. Additionally, SorCS1 regulates inflammatory responses by controlling cytokine receptor trafficking, suggesting roles in immune-related conditions.
Recombinant SorCS1 proteins, typically produced in mammalian or insect cell systems, retain functional domains for in vitro studies. These proteins are used to investigate ligand-receptor interactions, signaling mechanisms, and therapeutic targeting. For example, recombinant SorCS1 extracellular domains help map binding partners like proNGF or APP, while truncated forms aid in structural studies. Its dual roles in neurobiology and metabolism make SorCS1 a compelling target for understanding disease mechanisms and developing biologics. However, functional complexity due to alternative splicing and tissue-specific isoforms necessitates careful experimental design in research applications.
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