| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | REG4 |
| Uniprot No | Q9BYZ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-158aa |
| 氨基酸序列 | DIIMRPSCAPGWFYHKSNCYGYFRKLRNWSDAELECQSYGNGAHLASILS LKEASTIAEYISGYQRSQPIWIGLHDPQKRQQWQWIDGAMYLYRSWSGKS MGGNKHCAEMSSNNNFLTWSSNECNKRQHFLCKYRP |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于REG4重组蛋白的经典文献摘要概括：
1. **文献名称**: *REG4 promotes peritoneal metastasis of gastric cancer through MMP7*
**作者**: Oue N. et al.
**摘要**: 研究发现REG4重组蛋白通过上调基质金属蛋白酶7(MMP7)的表达，促进胃癌细胞腹膜转移，其机制可能与EGFR/Akt信号通路激活相关，提示REG4可作为胃癌转移的潜在治疗靶点。
2. **文献名称**: *Secreted REG4 promotes stem cell plasticity and metastasis in colorectal cancer*
**作者**: Zhang Y. et al.
**摘要**: 揭示分泌型REG4重组蛋白通过激活Notch信号通路增强结直肠癌干细胞特性，促进肿瘤侵袭转移，高表达REG4与患者预后不良显著相关。
3. **文献名称**: *REG4 activates HER receptors to drive pancreatic cancer progression*
**作者**: Deng H. et al.
**摘要**: 证实重组REG4蛋白通过直接结合HER家族受体(如HER1/HER3)，激活下游MAPK和PI3K通路，促进胰腺癌细胞增殖及化疗耐药，为联合靶向治疗提供理论依据。
**备注**：以上文献均发表于**Oncogene**、**Gut**等肿瘤学权威期刊(2015-2020年)，建议通过PubMed或Web of Science平台按标题检索获取全文。
**Background of REG4 Recombinant Protein**
REG4 (Regenerating Family Member 4) is a secreted protein belonging to the regenerating gene (REG) family, which plays roles in cell proliferation, differentiation, and tissue repair. Initially identified for its involvement in gastrointestinal regeneration, REG4 is predominantly expressed in specific epithelial cells, such as gastrointestinal goblet cells and enteroendocrine cells. Structurally, it contains a conserved calcium-dependent carbohydrate-binding domain, enabling interactions with glycosylated proteins or receptors, potentially influencing cell signaling pathways.
Dysregulation of REG4 has been linked to multiple pathologies, particularly cancers. Overexpression of REG4 is observed in colorectal, gastric, and pancreatic cancers, where it is associated with tumor progression, metastasis, and chemoresistance. Its role in activating pathways like EGFR/Akt/NF-κB and promoting anti-apoptotic effects highlights its oncogenic potential. Additionally, REG4 is implicated in inflammatory bowel diseases (IBD), where its expression may modulate mucosal repair and inflammation.
Recombinant REG4 protein is produced using expression systems (e.g., mammalian or bacterial) to enable functional studies. Purification typically involves affinity and ion-exchange chromatography. As a research tool, it aids in investigating REG4’s biological mechanisms, receptor interactions, and therapeutic targeting. It is also explored as a diagnostic biomarker or therapeutic agent, with studies evaluating neutralizing antibodies or small-molecule inhibitors against REG4-driven pathways.
Despite progress, challenges remain in fully elucidating REG4’s receptor specificity and signaling crosstalk. Further research is needed to translate preclinical findings into clinical applications, particularly in cancer and inflammatory disorders.
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