| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DATF1 |
| Uniprot No | Q9BTC0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-544aa |
| 氨基酸序列 | MDDKGDPSNEEAPKAIKPTSKEFRKTWGFRRTTIAKREGAGDAEADPLEPPPPQQQLGLSLRRSGRQPKRTERVEQFLTIARRRGRRSMPVSLEDSGEPTSCPATDAETASEGSVESASETRSGPQSASTAVKERPASSEKVKGGDDHDDTSDSDSDGLTLKELQNRLRRKREQEPTERPLKGIQSRLRKKRREEGPAETVGSEASDTVEGVLPSKQEPENDQGVVSQAGKDDRESKLEGKAAQDIKDEEPGDLGRPKPECEGYDPNALYCICRQPHNNRFMICCDRCEEWFHGDCVGISEARGRLLERNGEDYICPNCTILQVQDETHSETADQQEAKWRPGDADGTDCTSIGTIEQKSSEDQGIKGRIEKAANPSGKKKLKIFQPVIEAPGASKCIGPGCCHVAQPDSVYCSNDCILKHAAATMKFLSSGKEQKPKPKEKMKMKPEKPSLPKCGAQAGIKISSVHKRPAPEKKETTVKKAVVVPARSEALGKEAACESSTPSWASDHNYNAVKPEKTAAPSPSLLYKCSGKYLYSLHPSLIA |
| 分子量 | 85.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于可能的关键词调整后整理的3篇相关文献示例，供参考：
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1. **文献名称**: *Recombinant expression and functional analysis of a novel apoptosis-inducing protein DATF1 in human glioblastoma cells*
**作者**: Zhang Y, et al.
**摘要**: 研究报道了重组人DATF1蛋白在大肠杆菌中的可溶性表达及纯化，并验证了其在胶质母细胞瘤细胞中诱导凋亡的功能，表明DATF1可能通过激活caspase-3通路抑制肿瘤生长。
2. **文献名称**: *DATF1 modulates oxidative stress resistance via regulating Nrf2 signaling pathway*
**作者**: Li H, Wang X, et al.
**摘要**: 通过哺乳动物细胞系统表达重组人DATF1.发现其能增强细胞对H₂O₂诱导的氧化应激的抗性，机制涉及调控Nrf2核转位及下游抗氧化基因表达。
3. **文献名称**: *Characterization of recombinant human DATF1 as a potential therapeutic target in autoimmune diseases*
**作者**: Chen L, et al.
**摘要**: 研究利用HEK293细胞表达重组DATF1蛋白，发现其可抑制T细胞过度活化，减轻小鼠模型中的类风湿性关节炎症状，提示其免疫调节应用潜力。
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**说明**：由于“DATF1”可能为特定研究中的命名，以上文献为虚拟示例。若实际检索困难，建议进一步确认蛋白名称或扩展检索词(如“DATF1 apoptosis”“recombinant expression”)。如需真实文献，请提供更多蛋白背景或验证名称拼写。
**Recombinant Human DATF1 Protein: Background Overview**
The human DATF1 (Death Associated Transcription Factor 1) protein, also known as BCLAF1. is a multifunctional nuclear protein implicated in regulating apoptosis, transcriptional control, and DNA damage response. It interacts with key signaling pathways, including p53 and NF-κB, to modulate cell survival and death under stress conditions. Structurally, DATF1 contains domains for DNA binding and protein interactions, enabling its role in transcriptional repression or activation depending on cellular context.
Recombinant DATF1 protein is engineered using *in vitro* expression systems (e.g., *E. coli* or mammalian cell lines) to produce purified, bioactive forms for research. Its recombinant version allows precise study of molecular mechanisms, such as how DATF1 binds DNA, recruits chromatin modifiers, or influences apoptosis-related genes like Bcl-2 family members. Studies highlight its dual role: promoting apoptosis in some cancers while supporting survival in others, underscoring context-dependent functionality.
Research on recombinant DATF1 is critical for exploring its potential as a therapeutic target, particularly in oncology and neurodegenerative diseases. Dysregulation of DATF1 is linked to tumor progression, chemotherapy resistance, and aberrant cell cycle regulation. Additionally, its involvement in splicing and mRNA processing broadens its relevance in cellular homeostasis. Ongoing studies aim to clarify its regulatory networks and therapeutic applications, leveraging recombinant protein tools to dissect its complex biological roles.
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