| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DACH1 |
| Uniprot No | Q9UI36 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-558aa |
| 氨基酸序列 | MAVPAALIPPTQLVPPQPPISTSASSSGTTTSTSSATSSPAPSIGPPASSGPTLFRPEPIASAAAAAATVTSTGGGGGGGGSGGGGGSSGNGGGGGGGGGGSNCNPNLAAASNGSGGGGGGISAGGGVASSTPINASTGSSSSSSSSSSSSSSSSSSSSSSSSCGPLPGKPVYSTPSPVENTPQNNECKMVDLRGAKVASFTVEGCELICLPQAFDLFLKHLVGGLHTVYTKLKRLEITPVVCNVEQVRILRGLGAIQPGVNRCKLISRKDFETLYNDCTNASSRPGRPPKRTQSVTSPENSHIMPHSVPGLMSPGIIPPTGLTAAAAAAAAATNAAIAEAMKVKKIKLEAMSNYHASNNQHGADSENGDMNSSVDETPLSTPTARDSLDKLSLTGHGQPLPPGFPSPFLFPDGLSSIETLLTNIQGLLKVAIDNARAQEKQVQLEKTELKMDFLRERELRETLEKQLAMEQKNRAIVQKRLKKEKKAKRKLQEALEFETKRREQAEQTLKQAASTDSLRVLNDSLTPEIEADRSGGRTDAERTIQDGRLYLKTTVMY |
| 分子量 | 61.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DACH1蛋白的3篇代表性文献概览：
1. **"DACH1 inhibits TGF-β-induced epithelial-mesenchymal transition and metastasis in breast cancer"**
- **作者**: Wu et al. (2019)
- **摘要**: 该研究通过表达重组人DACH1蛋白，揭示其通过结合Smad4抑制TGF-β信号通路，从而阻止乳腺癌细胞的上皮-间充质转化(EMT)和转移，为靶向治疗提供新思路。
2. **"DACH1 suppresses tumor progression by regulating HER2/cyclin D1 signaling"**
- **作者**: Pawar et al. (2016)
- **摘要**: 实验通过重组DACH1蛋白验证其作为HER2转录抑制因子的功能，发现其能下调Cyclin D1表达，抑制乳腺癌细胞增殖并阻滞细胞周期于G1期，阐明其抗肿瘤机制。
3. **"Recombinant DACH1 protein inhibits prostate cancer growth by antagonizing the androgen receptor"**
- **作者**: Xie et al. (2018)
- **摘要**: 研究者利用纯化的重组DACH1蛋白证明其可直接结合雄激素受体(AR)，干扰AR与靶基因启动子的相互作用，抑制前列腺癌细胞增殖及移植瘤生长，提出其在前列腺癌治疗中的潜在价值。
**备注**：以上文献为虚拟示例，具体研究需通过PubMed或Web of Science检索关键词“recombinant DACH1 protein”、“DACH1 cancer function”获取。
**Background of Recombinant Human DACH1 Protein**
DACH1 (Dachshund homolog 1) is a conserved transcription regulator belonging to the DACH protein family, which plays critical roles in embryonic development, cell fate determination, and tumorigenesis. Initially identified as a key player in eye, limb, and brain development, DACH1 interacts with DNA-binding proteins and chromatin modifiers to modulate gene expression. It functions as a tumor suppressor in certain cancers (e.g., breast, prostate) by antagonizing oncogenic signaling pathways, such as TGF-β/Smad and Wnt/β-catenin. However, its role is context-dependent, as it may also promote tumor progression in specific malignancies.
Recombinant human DACH1 protein is engineered using molecular cloning techniques, typically expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. This purified protein serves as a vital tool for studying DACH1's molecular mechanisms, including its interactions with binding partners (e.g., Six1. NuRD complex) and regulatory effects on target genes. Researchers utilize it to explore its dual roles in cancer, tissue regeneration, and developmental disorders. Additionally, recombinant DACH1 aids in drug discovery, particularly in designing therapies targeting its tumor-suppressive pathways or aberrant activity in diseases. Its structural and functional characterization continues to advance our understanding of cell differentiation, senescence, and cancer biology.
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