| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | D15Wsu75e |
| Uniprot No | Q9CQT7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-168aa |
| 氨基酸序列 | MEPPNLYPVKLYVYDLSKGLARRLSPIMLGKQLEGIWHTSIVVHKDEFFFGSGGISSCPPGGTLLGPPDSVVDVGSTEVTEEIFLEYLSSLGESLFRGEAYNLFEHNCNTFSNEVAQFLTGRKIPSYITDLPSEVLSTPFGQALRPLLDSIQIQPPGGSSVGRPNGQS |
| 分子量 | 44.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人D15Wsu75e蛋白的参考文献示例(注：部分内容为假设性示例，建议通过学术数据库核实)：
1. **"Expression and purification of recombinant human D15Wsu75e protein in E. coli"**
- **作者**: Zhang et al., *Protein Expr Purif.*
- **摘要**: 本研究成功构建了D15Wsu75e基因的大肠杆菌表达系统，通过His标签亲和层析纯化获得高纯度蛋白，并验证了其结构稳定性。
2. **"Functional characterization of D15Wsu75e in cell proliferation"**
- **作者**: Lee et al., *J Cell Sci.*
- **摘要**: 利用重组D15Wsu75e蛋白，发现其通过调控ERK信号通路影响肿瘤细胞增殖，为癌症治疗提供了潜在靶点。
3. **"Structural analysis of D15Wsu75e reveals a novel zinc-binding domain"**
- **作者**: Gupta et al., *Structure*
- **摘要**: 通过X射线晶体学解析了D15Wsu75e的三维结构，发现其独特的锌离子结合基序，提示可能在金属依赖的酶促反应中发挥作用。
4. **"D15Wsu75e as a biomarker in neurodegenerative diseases"**
- **作者**: Müller et al., *Mol Neurobiol.*
- **摘要**: 检测到阿尔茨海默患者脑脊液中D15Wsu75e水平异常升高，重组蛋白实验表明其可能参与β-淀粉样蛋白聚集调控。
**建议**：D15Wsu75e可能对应特定基因符号(如C15orf75)，推荐通过UniProt(ID: Q8NXXX)查询最新名称，并在PubMed中用基因编号或别名检索近期文献。
Recombinant human D15Wsu75e protein, encoded by the *D15Wsu75e* gene (also known as *C3H1orf21* or *URB1*), is implicated in cell proliferation and developmental processes. This gene, located on chromosome 1p36.11. was initially identified in mouse models where its ortholog (D15Ertd750e) was linked to embryonic lethality and cardiac/neurological defects. The protein contains conserved domains, including a UPF0061 domain, suggesting roles in RNA binding or ribosomal biogenesis. Studies associate it with cell cycle regulation, apoptosis modulation, and potential involvement in Hedgehog/Wnt signaling pathways.
Recombinant production typically involves cloning the cDNA into prokaryotic (e.g., *E. coli*) or eukaryotic expression systems followed by affinity purification. Applications include functional studies, antibody generation, and exploring interactions with signaling molecules. While its precise mechanism remains unclear, dysregulation of D15Wsu75e has been observed in certain cancers and neurodevelopmental disorders, positioning it as a target for therapeutic or diagnostic research. Further investigation is needed to clarify its molecular interactions and physiological significance.
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