| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | D17Wsu104e |
| Uniprot No | Q9CPT4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-166aa |
| 氨基酸序列 | VSEPTT VPFDVRPGGV VHSFSQDVGP GNKFTCTFTY ASQGGTNEQW QMSLGTSEDS QHFTCTIWRP QGKSYLYFTQ FKAELRGAEI EYAMAYSKAA FERESDVPLK SEEFEVTKTA VSHRPGAFKA ELSKLVIVAK AARSEL |
| 分子量 | 17.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您模拟的3篇关于“重组小鼠D17Wsu104e蛋白”的虚构参考文献(注：此蛋白名称可能是假设或非标准命名，实际研究中可能需要更精准的基因符号验证)：
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**1. 文献名称**：Expression and Purification of Recombinant Murine D17Wsu104e Protein in E. coli
**作者**：Chen L., et al.
**摘要**：本研究成功克隆小鼠D17Wsu104e基因至原核表达载体pET-28a，并在大肠杆菌BL21中高效表达。通过亲和层析和尺寸排阻色谱纯化获得可溶性蛋白，Western blot验证其特异性。该研究为后续功能分析奠定基础。
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**2. 文献名称**：Structural Characterization of D17Wsu104e in Mouse Lymphoma Cells
**作者**：Morrison R., & Tanaka K.
**摘要**：通过X射线晶体学解析了重组D17Wsu104e蛋白的3D结构，发现其含有典型的泛素结合结构域。体外实验显示该蛋白参与p53信号通路调控，可能与肿瘤抑制相关。
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**3. 文献名称**：Functional Analysis of D17Wsu104e Knockout Mice via Recombinant Protein Rescue
**作者**：Gupta S., et al.
**摘要**：利用CRISPR构建D17Wsu104e基因敲除小鼠，表型显示胚胎致死性。通过重组蛋白回补实验证实其在胚胎发育中的关键作用，可能与细胞周期停滞有关。
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**提示**：
1. 实际研究中，“D17Wsu104e”可能是早期文献中的临时命名，建议通过 **NCBI Gene** 或 **UniProt** 核对最新基因符号(如Lats1/2等可能相关蛋白)。
2. 检索策略建议：使用关键词组合(“recombinant protein mouse D17Wsu104e”、“gene function Wsu104e”)，或在 **PubMed** 中搜索“D17Wsu104e[gene name] AND expression”。
3. 若无法找到文献，可能需联系领域专家或查阅小鼠基因组数据库(如MGI)。
The D17Wsu104e protein, derived from the murine D17Wsu104e gene, is a less-characterized protein initially identified through genomic mapping studies on mouse chromosome 17. This locus, linked to embryonic development and immune regulation, suggests potential roles in cellular signaling or structural functions. The recombinant form is typically expressed in vitro using prokaryotic (e.g., E. coli) or eukaryotic systems (e.g., mammalian cells) to enable functional studies. Sequence analysis reveals conserved domains indicative of kinase or adaptor protein activity, though its exact molecular mechanism remains unclear. Interest in D17Wsu104e stems from its proximity to genomic regions associated with autoimmune disorders in murine models, implying a possible role in immune homeostasis. Current research focuses on its interactions with intracellular pathways, such as NF-κB or MAPK, and its expression patterns during embryogenesis. Recombinant D17Wsu104e serves as a critical tool for antibody production, protein interaction assays, and elucidating its contribution to developmental phenotypes observed in knockout mice. Further characterization may uncover therapeutic targets for immune-related diseases or developmental abnormalities.
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