| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | D4S234E |
| Uniprot No | B2R5R8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-185aa |
| 氨基酸序列 | MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPQIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVVYKVYKYDRACPDGFVLKNTQCIPEGLESYYAEQDSSAREKFYTVINHYNLAKQSITRSVSPWMSVLSEEKLSEQETEAAEKSA |
| 分子量 | 46.09 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇模拟的关于重组人D4S234E蛋白的虚构参考文献(请注意这些文献为假设性示例，实际引用需查询真实数据库)：
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1. **文献名称**: "Expression and Functional Characterization of Recombinant Human D4S234E Protein in Neuronal Cells"
**作者**: Zhang L., et al.
**摘要**: 本研究通过杆状病毒表达系统成功表达重组人D4S234E蛋白，发现其通过与突触前膜蛋白相互作用，调控神经递质释放，提示其在神经信号传导中的潜在作用。
2. **文献名称**: "Structural Insights into the D4S234E Protein: Implications for Chromatin Remodeling"
**作者**: Thompson R., & Gupta S.
**摘要**: 利用X射线晶体学解析了重组人D4S234E蛋白的三维结构，揭示了其锌指结构域与DNA结合的特性，并证明其参与染色质重构及基因表达调控通路。
3. **文献名称**: "D4S234E Recombinant Protein as a Biomarker in Breast Cancer: Purification and Clinical Validation"
**作者**: Chen W., et al.
**摘要**: 开发了基于HEK293细胞的重组D4S234E蛋白纯化工艺，并通过临床样本验证其在乳腺癌组织中异常高表达，提示其作为肿瘤诊断标志物的潜力。
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**说明**：以上文献为根据领域常见研究方向模拟的内容，实际研究中建议通过PubMed、Web of Science等平台检索真实文献(关键词如“recombinant D4S234E protein”或“D4S234E functional study”)。
**Background of Recombinant Human D4S234E Protein**
The D4S234E protein, encoded by the *D4S234E* gene (previously known as *LRRC36*), is a member of the leucine-rich repeat (LRR) protein family, characterized by conserved structural motifs involved in protein-protein interactions. Although its exact physiological function remains partially characterized, bioinformatics analyses suggest potential roles in cellular signaling, immune regulation, or extracellular matrix organization. The gene is located on chromosome 4 (4p16.3) and has been implicated in studies exploring genetic associations with neurodevelopmental disorders and cancer progression.
Recombinant human D4S234E protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its production typically involves cloning the cDNA sequence into expression vectors, followed by purification via affinity chromatography. This recombinant form allows researchers to investigate the protein's structure, binding partners, and activity in vitro or in cell-based assays. Emerging research highlights its potential interaction with components of the TGF-β signaling pathway or cytoskeletal proteins, though mechanistic insights are limited.
Current applications include its use as an antigen for antibody development or in high-throughput screening to identify modulators of its function. Despite progress, the protein's full biological significance and therapeutic relevance await further exploration through targeted knockout models and disease association studies.
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