| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DAZ1 |
| Uniprot No | Q9NQZ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-744aa |
| 氨基酸序列 | MSAANPETPN STISREASTQ SSSAAASQGW VLPEGKIVPN TVFVGGIDAR MDETEIGSCF GRYGSVKEVK IITNRTGVSK GYGFVSFVND VDVQKIVGSQ IHFHGKKLKL GPAIRKQKLC ARHVQPRPLV VNPPPPPQFQ NVWRNPNTET YLQPQITPNP VTQHVQSAAN PETPNSTISR EASTQSSSAA ASQGWVLPEG KIVPNTVFVG GIDARMDETE IGSCFGRYGS VKEVKIITNR TGVSKGYGFV SFVNDVDVQK IVGSQIHFHG KKLKLGPAIR KQKLCARHVQ PRPLVVNPPP PPQFQNVWRN PNTETYLQPQ ITPNPVTQHV QSAANPETPN STISREASTQ SSSAAASQGW VLPEGKIVPN TVFVGGIDAR MDETEIGSCF GRYGSVKEVK IITNRTGVSK GYGFVSFVND VDVQKIVGSQ IHFHGKKLKL GPAIRKQKLC ARHVQPRPLV VNPPPPPQFQ NVWRNPNTET YLQPQITPNP VTQHVQAYSA YPHSPGQVIT GCQLLVYNYQ EYPTYPDSAF QVTTGYQLPV YNYQPFPAYP RSPFQVTAGY QLPVYNYQAF PAYPNSPFQV ATGYQFPVYN YQPFPAYPSS PFQVTAGYQL PVYNYQAFPA YPNSPFQVAT GYQFPVYNYQ AFPAYPNSPV QVTTGYQLPV YNYQAFPAYP SSPFQVTTGY QLPVYNYQAF PAYPNSAVQV TTGYQFHVYN YQMPPQCPVG EQRRNLWTEA YKWWYLVCLI QRRD |
| 分子量 | 82.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DAZ1蛋白的示例文献(虚构内容，仅作格式参考)：
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1. **文献名称**：Recombinant Expression and Functional Analysis of Human DAZ1 Protein in E. coli
**作者**：Zhang et al.
**摘要**：本研究在大肠杆菌中成功表达了重组人DAZ1蛋白，并通过亲和层析纯化获得高纯度产物。实验验证了DAZ1的RNA结合能力，并发现其能特异性识别睾丸特异性mRNA，提示其在精子发生中的调控作用。
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2. **文献名称**：Structural Characterization of DAZ1 Protein and Its Interaction with PUM2
**作者**：Brown & Smith
**摘要**：通过X射线晶体学解析了重组DAZ1蛋白的结构，揭示了其RNA识别基序(RRM)的构象。进一步实验证明DAZ1与PUM2蛋白在生殖细胞中形成复合物，协同调控减数分裂相关基因的表达。
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3. **文献名称**：DAZ1 Knockdown and Rescue in a Human Germ Cell Model
**作者**：Li et al.
**摘要**：利用CRISPR技术构建DAZ1敲除的人类生殖细胞系，并通过外源重组DAZ1蛋白回补实验证明其可恢复精子发生关键通路活性，为男性不育的机制研究提供了新证据。
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4. **文献名称**：Clinical Implications of DAZ1 Autoantibodies in Infertility
**作者**：Wang et al.
**摘要**：在部分不明原因不育男性血清中检测到DAZ1自身抗体。研究证实重组DAZ1蛋白可作为诊断抗原，其抗体水平与精子质量下降显著相关，为免疫性不育提供了潜在标志物。
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注：以上文献为示例，实际研究中需检索真实数据库(如PubMed)获取具体信息。
Recombinant human DAZ1 protein is a genetically engineered version of the DAZ1 (Deleted in Azoospermia 1) protein, a critical factor in male germ cell development. DAZ1 belongs to the DAZ gene family, located on the Y chromosome, and is predominantly expressed in germ cells, particularly during spermatogenesis. It encodes an RNA-binding protein containing a conserved RNA recognition motif (RRM) and a DAZ motif, which are essential for regulating mRNA translation and stability in developing sperm cells. Mutations or deletions in DAZ1 are strongly associated with male infertility, often leading to azoospermia or severe oligozoospermia.
The recombinant form of DAZ1 is typically produced in heterologous expression systems (e.g., E. coli or mammalian cells) to enable functional studies. Its production allows researchers to investigate DAZ1's molecular mechanisms in germ cell maturation, including its role in meiosis, spermatid differentiation, and interaction with partner proteins like BOLL and PUM2. Recombinant DAZ1 also serves as a tool for developing diagnostic assays or therapeutic strategies for infertility. Studies using this protein have provided insights into conserved fertility pathways across species, bridging gaps between basic reproductive biology and clinical applications. However, challenges remain in fully recapitulating its post-translational modifications and in vivo interactions using synthetic systems.
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