| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DENND1C |
| Uniprot No | Q8IV53 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-801aa |
| 氨基酸序列 | MESRAEGGSPAVFDWFFEAACPASLQEDPPILRQFPPDFRDQEAMQMVPKFCFPFDVEREPPSPAVQHFTFALTDLAGNRRFGFCRLRAGTQSCLCILSHLPWFEVFYKLLNTVGDLLAQDQVTEAEELLQNLFQQSLSGPQASVGLELGSGVTVSSGQGIPPPTRGNSKPLSCFVAPDSGRLPSIPENRNLTELVVAVTDENIVGLFAALLAERRVLLTASKLSTLTSCVHASCALLYPMRWEHVLIPTLPPHLLDYCCAPMPYLIGVHASLAERVREKALEDVVVLNVDANTLETTFNDVQALPPDVVSLLRLRLRKVALAPGEGVSRLFLKAQALLFGGYRDALVCSPGQPVTFSEEVFLAQKPGAPLQAFHRRAVHLQLFKQFIEARLEKLNKGEGFSDQFEQEITGCGASSGALRSYQLWADNLKKGGGALLHSVKAKTQPAVKNMYRSAKSGLKGVQSLLMYKDGDSVLQRGGSLRAPALPSRSDRLQQRLPITQHFGKNRPLRPSRRRQLEEGTSEPPGAGTPPLSPEDEGCPWAEEALDSSFLGSGEELDLLSEILDSLSMGAKSAGSLRPSQSLDCCHRGDLDSCFSLPNIPRWQPDDKKLPEPEPQPLSLPSLQNASSLDATSSSKDSRSQLIPSESDQEVTSPSQSSTASADPSIWGDPKPSPLTEPLILHLTPSHKAAEDSTAQENPTPWLSTAPTEPSPPESPQILAPTKPNFDIAWTSQPLDPSSDPSSLEDPRARPPKALLAERAHLQPREEPGALNSPATPTSNCQKSQPSSRPRVADLKKCFEG |
| 分子量 | 113.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DENND1C蛋白的模拟参考文献示例(仅供格式参考，非真实文献)：
1. **《DENND1C regulates endosomal trafficking and extracellular vesicle secretion in cancer cells》**
- 作者：Smith A, et al.
- 摘要：研究重组DENND1C蛋白通过激活Rab35调控晚期内体分选和癌细胞外泌体释放的机制，揭示其在肿瘤转移中的作用。
2. **《Structural and functional characterization of the DENND1C GEF domain》**
- 作者：Chen L, et al.
- 摘要：通过重组表达人DENND1C的GEF结构域，解析其晶体结构，证实其作为鸟苷酸交换因子调控Rab蛋白活性的分子基础。
3. **《DENND1C deficiency disrupts adipocyte differentiation via vesicle trafficking impairment》**
- 作者：Wang Y, et al.
- 摘要：利用重组DENND1C蛋白恢复脂肪细胞分化模型，证明其通过调控Rab蛋白依赖的囊泡运输影响脂滴形成和代谢通路。
4. **《Recombinant DENND1C expression in HEK293 cells: purification and interaction screening》**
- 作者：Zhang R, et al.
- 摘要：优化重组人DENND1C在哺乳动物细胞中的表达与纯化流程，并通过蛋白质互作组学鉴定其结合伴侣，为功能研究提供工具。
**提示**：实际文献可通过PubMed或Google Scholar以关键词“recombinant DENND1C”“DENND1C function”等查询。
Recombinant human DENND1C protein is a genetically engineered form of the DENND1C (Differentially Expressed in Normal and Neoplastic Cells Domain 1C) protein, a member of the DENN domain-containing protein family. DENND1C, also known as connecdenn 3. functions as a guanine nucleotide exchange factor (GEF) for Rab35 GTPases, which are critical regulators of intracellular membrane trafficking, endocytosis, and cytoskeletal dynamics. The protein contains conserved DENN and dDENN domains that mediate interactions with Rab GTPases, facilitating their activation and subsequent roles in vesicle transport and signaling pathways.
DENND1C is implicated in neuronal development, immune regulation, and secretory processes in endocrine cells. Its recombinant form is commonly produced in bacterial or mammalian expression systems for functional studies, enabling research into its role in cellular pathways and disease contexts. Altered DENND1C expression or mutations have been linked to neurodevelopmental disorders and cancer, making it a potential therapeutic target. Recombinant DENND1C allows biochemical characterization, antibody development, and high-throughput screening, advancing insights into Rab-mediated trafficking mechanisms and their pathological disruptions.
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