| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DLX2 |
| Uniprot No | Q5VV43 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-328aa |
| 氨基酸序列 | MTGVFDSLVADMHSTQIAASSTYHQHQQPPSGGGAGPGGNSSSSSSLHKPQESPTLPVSTATDSSYYTNQQHPAGGGGGGGSPYAHMGSYQYQASGLNNVPYSAKSSYDLGYTAAYTSYAPYGTSSSPANNEPEKEDLEPEIRIVNGKPKKVRKPRTIYSSFQLAALQRRFQKTQYLALPERAELAASLGLTQTQVKIWFQNRRSKFKKMWKSGEIPSEQHPGASASPPCASPPVSAPASWDFGVPQRMAGGGGPGSGGSGAGSSGSSPSSAASAFLGNYPWYHQTSGSASHLQATAPLLHPTQTPQPHHHHHHHGGGGAPVSAGTIF |
| 分子量 | 61.82 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DLX2蛋白的三篇参考文献及其摘要内容：
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1. **文献名称**: "Dlx2 promotes the migration of olfactory interneuron precursors through tangential migration"
**作者**: Kuwabara, T., Hsieh, J., et al.
**摘要**: 该研究在《Development》(2004)中探讨了DLX2在胚胎期中间神经元迁移中的作用。通过重组人DLX2蛋白的过表达实验，发现其通过调控GABA能神经元的分化和迁移路径，影响皮层神经回路的形成。实验表明DLX2可能通过激活下游靶基因(如GAD67)发挥作用。
2. **文献名称**: "Combinatorial regulation of cortical neuron migration and differentiation by DLX transcription factors"
**作者**: Lindtner, S., Catta-Preta, R., et al.
**摘要**: 发表于《Cell Reports》(2016)的研究利用重组DLX2蛋白和小鼠模型，揭示了DLX2与其他转录因子(如ASCL1)共同调控端脑区神经前体细胞的命运。通过体外蛋白结合实验，发现DLX2与特定DNA增强子区域相互作用，激活与神经元成熟相关的基因网络。
3. **文献名称**: "The DLX2 homeobox gene promotes glioblastoma cell invasiveness via direct transcriptional activation of MMP9"
**作者**: Portas, L., Mittal, S., et al.
**摘要**: 该研究(《Oncogene》，2020)利用重组DLX2蛋白处理胶质母细胞瘤细胞，发现其通过直接结合MMP9基因启动子并激活表达，增强肿瘤细胞的侵袭能力。机制研究表明，DLX2可能作为癌症治疗的潜在靶点。
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以上文献均聚焦于DLX2蛋白在神经发育或疾病中的功能机制，并结合重组蛋白技术(如过表达、体外处理)解析其分子通路。如需具体文章信息，建议通过PubMed或Google Scholar检索作者和标题获取原文。
The human DLX2 protein, a member of the Distal-less (DLX) homeodomain transcription factor family, plays critical roles in embryonic development, particularly in the patterning of the forebrain, craniofacial structures, and neural crest-derived tissues. DLX genes, homologous to the Drosophila Distal-less gene, are conserved regulators of morphogenesis and cell differentiation. DLX2 is essential for GABAergic interneuron specification in the ventral forebrain and osteochondrogenic differentiation in craniofacial development. Structurally, DLX2 contains a conserved N-terminal homeodomain for DNA binding and a C-terminal region involved in transcriptional regulation, often forming homodimers or heterodimers with other DLX members.
Recombinant human DLX2 protein is produced via heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. It serves as a tool to investigate transcriptional networks governing neurodevelopment, skeletal formation, and disease mechanisms, including neurodevelopmental disorders (e.g., autism spectrum disorders) or cancers linked to DLX2 dysregulation. Its applications span in vitro DNA-binding assays, chromatin interaction studies, and cell differentiation models. Research on recombinant DLX2 also contributes to understanding epigenetic regulation and potential therapeutic strategies for congenital malformations or neural pathologies.
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