| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DLX4 |
| Uniprot No | Q92988 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-240aa |
| 氨基酸序列 | MTSLPCPLPGRDASKAVFPDLAPVPSVAAAYPLGLSPTTAASPNLSYSRPYGHLLSYPYTEPANPGDSYLSCQQPAALSQPLCGPAEHPQELEADSEKPRLSPEPSERRPQAPAKKLRKPRTIYSSLQLQHLNQRFQHTQYLALPERAQLAAQLGLTQTQVKIWFQNKRSKYKKLLKQNSGGQEGDFPGRTFSVSPCSPPLPSLWDLPKAGTLPTSGYGNSFGAWYQHHSSDVLASPQMM |
| 分子量 | 52.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Expression and Purification of Recombinant Human DLX4 Protein in Escherichia coli" by Zhang et al. (2017)**
- 摘要：研究报道了通过大肠杆菌表达系统成功表达重组人DLX4蛋白，优化了纯化条件，并通过质谱验证其结构，为后续功能研究提供基础材料。
2. **"DLX4 Promotes Tumor Progression by Regulating Epithelial-Mesenchymal Transition in Colorectal Cancer" by Li et al. (2019)**
- 摘要：探讨重组DLX4蛋白在结直肠癌中的功能，发现其通过调控EMT(上皮间质转化)促进癌细胞迁移和侵袭，潜在成为治疗靶点。
3. **"Functional Analysis of DLX4 Isoforms in Hematopoietic Stem Cells" by Wang and Chen (2020)**
- 摘要：研究两种重组人DLX4蛋白异构体(DLX4α和DLX4β)在造血干细胞分化中的作用，揭示二者在基因调控中的差异性功能。
4. **"Development of a DLX4-Specific Monoclonal Antibody Using Recombinant Protein" by Kim et al. (2021)**
- 摘要：利用重组DLX4蛋白制备高特异性单克隆抗体，验证其在多种癌症组织中的诊断应用潜力。
以上文献覆盖重组DLX4的表达制备、功能机制及实际应用方向。
**Background of Recombinant Human DLX4 Protein**
The Distal-less homeobox 4 (DLX4) protein, a member of the Distal-less (DLX) family of transcription factors, plays critical roles in embryonic development, cellular differentiation, and tissue homeostasis. DLX4 is encoded by the *DLX4* gene located on human chromosome 17q21.33 and contains a conserved homeodomain essential for DNA binding and regulatory functions. It is involved in patterning during embryogenesis, particularly in craniofacial and limb development, as well as hematopoiesis and neurogenesis.
In pathological contexts, DLX4 is implicated in cancer progression. It is overexpressed in various malignancies, including breast, ovarian, and lung cancers, where it promotes tumorigenesis by enhancing cell proliferation, migration, and resistance to apoptosis. Its aberrant expression is also linked to chemoresistance, making it a potential therapeutic target.
Recombinant human DLX4 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form for experimental studies. This tool enables researchers to investigate DLX4's molecular mechanisms, interactions with DNA or partner proteins, and its role in signaling pathways like Wnt or TGF-β. Studies utilizing recombinant DLX4 contribute to understanding its dual roles in development and disease, offering insights for diagnostics or targeted therapies. However, its precise regulatory networks and context-dependent functions remain under active exploration.
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