| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DLX5 |
| Uniprot No | P56178 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-289aa |
| 氨基酸序列 | MTGVFDRRVPSIRSGDFQAPFQTSAAMHHPSQESPTLPESSATDSDYYSPTGGAPHGYCSPTSASYGKALNPYQYQYHGVNGSAGSYPAKAYADYSYASSYHQYGGAYNRVPSATNQPEKEVTEPEVRMVNGKPKKVRKPRTIYSSFQLAALQRRFQKTQYLALPERAELAASLGLTQTQVKIWFQNKRSKIKKIMKNGEMPPEHSPSSSDPMACNSPQSPAVWEPQGSSRSLSHHPHAHPPTSNQSPASSYLENSASWYTSAASSINSHLPPPGSLQHPLALASGTLY |
| 分子量 | 57.53 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DLX5蛋白的参考文献示例，内容基于学术文献的典型研究方向归纳，部分文献可能为假设性举例：
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1. **文献名称**: *DLX5 promotes osteogenic differentiation of human mesenchymal stem cells via the Wnt/β-catenin pathway*
**作者**: Li et al.
**摘要**: 本研究通过表达重组人DLX5蛋白，发现其通过激活Wnt/β-catenin信号通路促进人间充质干细胞的成骨分化，为骨再生治疗提供了潜在靶点。
2. **文献名称**: *Recombinant DLX5 protein induces neuronal differentiation in embryonic stem cells*
**作者**: Martinez et al.
**摘要**: 利用重组DLX5蛋白处理胚胎干细胞，证明其通过上调NeuroD1等转录因子驱动神经元特异性基因表达，提示DLX5在神经发育中的关键作用。
3. **文献名称**: *Structural and functional characterization of human DLX5 homeodomain*
**作者**: Kimura et al.
**摘要**: 对重组人DLX5蛋白的同源结构域进行结构解析，揭示了其与DNA结合的分子机制，并验证了其在调控下游靶基因(如Runx2)中的功能。
4. **文献名称**: *DLX5 modulates BMP signaling in craniofacial development through direct protein interaction*
**作者**: García-Ruiz et al.
**摘要**: 研究重组DLX5蛋白与BMP信号通路分子的相互作用，证明其通过结合Smad1/5调控颅面发育相关基因的表达。
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**注意**：上述文献为示例性内容，实际研究中可能需结合具体实验条件或疾病模型。建议通过PubMed、Google Scholar等平台以“recombinant DLX5 protein”“DLX5 expression and purification”等关键词检索最新文献。若研究聚焦特定领域(如骨骼、神经或癌症)，可进一步细化关键词。
Recombinant human DLX5 (rhDLX5) protein is a genetically engineered form of the DLX5 transcription factor, which belongs to the Distal-less (DLX) family of homeobox genes. DLX5 plays critical roles in embryonic development, particularly in craniofacial morphogenesis, skeletal formation, and neuronal differentiation. It regulates the expression of target genes involved in osteoblast differentiation, limb development, and neurogenesis by binding to specific DNA sequences. Dysregulation of DLX5 has been associated with congenital disorders, including craniosynostosis, and certain cancers. The recombinant protein is typically produced using expression systems like *E. coli* or mammalian cell cultures, ensuring high purity and activity for experimental applications. Researchers utilize rhDLX5 to study its molecular interactions, transcriptional regulatory mechanisms, and involvement in developmental pathways. It is also employed in disease modeling, drug screening, and exploring therapeutic strategies for bone regeneration or neurological disorders. Functional studies often involve in vitro assays (e.g., DNA-binding, chromatin immunoprecipitation) or cellular models to assess its role in differentiation and tissue repair. By providing a controlled and scalable source of DLX5. this recombinant tool bridges gaps in understanding developmental biology and disease pathogenesis.
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