| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CBX3 |
| Uniprot No | Q13185 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-183aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMASNKT TLQKMGKKQN GKSKKVEEAE PEEFVVEKVL DRRVVNGKVE YFLKWKGFTD ADNTWEPEEN LDCPELIEAF LNSQKAGKEK DGTKRKSLSD SESDDSKSKK KRDAADKPRG FARGLDPERI IGATDSSGEL MFLMKWKDSD EADLVLAKEA NMKCPQIVIA FYEERLTWHS CPEDEAQ |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CBX3重组蛋白的3篇代表性文献及其摘要概括：
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1. **"Structural basis for specific binding of human HP1 proteins to histone H3K9me3"**
*Authors: Hiromi Yamada, Damien Boeckers, et al.*
**摘要**：本研究通过重组表达人源CBX3(HP1γ)蛋白，解析了其染色质结合结构域与H3K9me3修饰的复合物晶体结构，揭示了其通过chromodomain特异性识别甲基化组蛋白的分子机制。
2. **"Recombinant CBX3 chromodomain as a tool for epigenetics drug discovery"**
*Authors: Laura M. Gagné, Jean-Philippe Lambert, et al.*
**摘要**：研究团队在大肠杆菌中高效表达并纯化了重组CBX3的chromodomain片段，开发了基于该蛋白的高通量筛选平台，用于筛选靶向HP1蛋白与H3K9me3相互作用的抑制剂。
3. **"Functional characterization of CBX3 in heterochromatin formation via in vitro reconstitution"**
*Authors: Tingting Zhang, Guohong Li, et al.*
**摘要**：通过重组CBX3蛋白与核小体的体外重构实验，证明CBX3通过结合H3K9me3招募SUV39H1甲基转移酶，促进异染色质的扩增和基因沉默。
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这些文献涵盖了CBX3重组蛋白的结构解析、药物筛选应用及功能机制研究，可作为相关领域的重要参考。建议通过PubMed或Web of Science根据标题和作者进一步检索全文。
CBX3 (Chromobox homolog 3), also known as HP1γ (Heterochromatin Protein 1 gamma), is a member of the Polycomb group (PcG) protein family that plays a critical role in epigenetic regulation. It is a component of the Polycomb repressive complex 1 (PRC1), which mediates gene silencing through chromatin modification. Structurally, CBX3 contains two conserved domains: an N-terminal chromodomain that recognizes and binds to trimethylated lysine 27 on histone H3 (H3K27me3), and a C-terminal chromoshadow domain involved in protein-protein interactions and heterochromatin formation.
Recombinant CBX3 protein is engineered for experimental studies to explore its biological functions, particularly in chromatin organization, transcriptional repression, and genome stability. It is commonly expressed in bacterial or mammalian systems with affinity tags (e.g., His, GST) to facilitate purification. Researchers use recombinant CBX3 to investigate its role in cellular processes such as X-chromosome inactivation, DNA repair, and stem cell differentiation. Dysregulation of CBX3 has been linked to cancer progression, neurodegenerative diseases, and developmental disorders, making it a target for therapeutic exploration.
In drug discovery, recombinant CBX3 serves as a tool to screen small molecules that modulate PRC1 activity or disrupt CBX3 interactions with chromatin. Its study also sheds light on the dynamics of epigenetic inheritance and the crosstalk between Polycomb proteins and other chromatin modifiers. Despite its established role in heterochromatin maintenance, emerging research highlights context-dependent functions in gene activation, underscoring the complexity of epigenetic regulation. Recombinant CBX3 thus remains a vital reagent for dissecting the molecular mechanisms of chromatin biology and disease.
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