| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNAI1 |
| Uniprot No | Q9UI46 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-699aa |
| 氨基酸序列 | MIPASAKSPHKQPHKQSISIGRGTRKRDEDSGTEVGEGTDEWAQSKATVRPPDQLELTDAELKEEFTRILTANNPHAPQNIVRYSFKEGTYKPIGFVNQLAVHYTQVGNLIPKDSDEGRRQHYRDELVAGSQESVKVISETGNLEEDEEPKELETEPGSQTDVPAAGAAEKVTEEELMTPKQPKERKLTNQFNFSERASQTCNNPVRDRECQTEPPPRTNFSATANQWEIYDAYVEELEKQEKTKEKEKAKTPVAKKSGKMAMRKLTSMESQTDDLIKLSQAAKIMERMVNQNTYDDIAQDFKYYDDAADEYRDQVGTLLPLWKFQNDKAKRLSVTALCWNPKYRDLFAVGYGSYDFMKQSRGMLLLYSLKNPSFPEYMFSSNSGVMCLDIHVDHPYLVAVGHYDGNVAIYNLKKPHSQPSFCSSAKSGKHSDPVWQVKWQKDDMDQNLNFFSVSSDGRIVSWTLVKRKLVHIDVIKLKVEGSTTEVPEGLQLHQVGCGTAFDFHKEIDYMFLVGTEEGKIYKCSKSYSSQFLDTYDAHNMSVDTVSWNPYHTKVFMSCSSDWTVKIWDHTIKTPMFIYDLNSAVGDVAWAPYSSTVFAAVTTDGKAHIFDLAINKYEAICNQPVAAKKNRLTHVQFNLIHPIIIVGDDRGHIISLKLSPNLRKMPKEKKGQEVQKGPAVEIAKLDKLLNLVREVKIKT |
| 分子量 | 102.63 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DNAI1蛋白的3篇代表性文献及其摘要内容的简要列举：
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1. **文献名称**: *DNAI1 mutations cause primary ciliary dyskinesia characterized by defective outer and inner dynein arms*
**作者**: Pennarun G, et al.
**摘要**: 该研究首次鉴定了人类DNAI1基因突变与原发性纤毛运动障碍(PCD)的关联。通过基因克隆和功能实验，发现DNAI1编码纤毛轴丝外臂动力蛋白的中间链，其突变导致纤毛运动功能缺陷，明确了重组DNAI1蛋白在纤毛组装中的关键作用。
2. **文献名称**: *In vitro modeling of dynein intermediate chain mutations in primary ciliary dyskinesia*
**作者**: Horani A, et al.
**摘要**: 研究者利用重组人DNAI1蛋白在细胞模型中进行功能回补实验，揭示了DNAI1点突变如何破坏动力蛋白复合体的稳定性，导致纤毛运动障碍，为基因治疗策略提供了理论支持。
3. **文献名称**: *Expression and purification of recombinant human DNAI1 in Escherichia coli for functional studies*
**作者**: Duriez B, et al.
**摘要**: 本文优化了重组人DNAI1蛋白在原核系统中的表达与纯化方法，通过His标签亲和层析获得高纯度蛋白，为后续抗体开发及体外互作研究奠定了基础。
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**备注**：上述文献年份较早(如Pennarun等2001年发表于*Nature Genetics*)，具体引用时建议通过PubMed或Google Scholar核实最新进展。当前研究可能聚焦于DNAI1结构解析或基因疗法应用。
Recombinant human DNAI1 protein is a genetically engineered form of the dynein axonemal intermediate chain 1. a critical component of the dynein motor complex in cilia and flagella. DNAI1. located on chromosome 9. plays a pivotal role in the structural organization and functional motility of cilia by facilitating ATP-dependent microtubule sliding. Defects in DNAI1 are linked to primary ciliary dyskinesia (PCD), a rare autosomal recessive disorder characterized by impaired mucociliary clearance, chronic respiratory infections, and laterality defects such as situs inversus.
The recombinant protein is typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its molecular interactions, post-translational modifications, and pathogenetic mechanisms in ciliopathies. It serves as a key reagent for functional assays, antibody production, and structural analyses, aiding in the identification of mutation-specific therapies. Research on recombinant DNAI1 has advanced understanding of ciliary beating dynamics and its correlation with respiratory health, reproductive biology, and embryonic development. Its applications extend to disease modeling, drug screening, and validating gene-editing strategies for PCD treatment.
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