| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DMRTA1 |
| Uniprot No | Q5VZB9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-504aa |
| 氨基酸序列 | MERSQCGSRD RGVSGRPHLA PGLVVAAPPP PSPALPVPSG MQVPPAFLRP PSLFLRAAAA AAAAAAATSG SGGCPPAPGL ESGVGAVGCG YPRTPKCARC RNHGVVSALK GHKRFCRWRD CACAKCTLIA ERQRVMAAQV ALRRQQAQEE SEARGLQRLL CSGLSWPPGG RASGGGGRAE NPQSTGGPAA GAALGLGALR QASGSATPAF EVFQQDYPEE KQEQKESKCE SCQNGQEELI SKSHQLYLGS SSRSNGVIGK QSIGSSISEY SNKPDSILSP HPGEQSGGEE SPRSLSSSDL ESGNESEWVK DLTATKASLP TVSSRPRDPL DILTKIFPNY RRSRLEGILR FCKGDVVQAI EQVLNGKEHK PDNRNLANSE ELENTAFQRA SSFSLAGIGF GTLGNKSAFS PLQTTSASYG GDSSLYGVNP RVGISPLRLA YSSAGRGLSG FMSPYLTPGL VPTLPFRPAL DYAFSGMIRD SSYLSSKDSI TCGRLYFRPN QDNP |
| 分子量 | 53.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DMRTA1蛋白的3篇参考文献示例(内容为虚构，供参考)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant Human DMRTA1 in Neural Stem Cell Differentiation*
**作者**：Smith, J. et al.
**摘要**：本研究利用大肠杆菌系统成功表达并纯化了重组人DMRTA1蛋白，发现其通过调控Wnt/β-catenin信号通路促进神经干细胞向神经元分化，为神经再生研究提供了新靶点。
2. **文献名称**：*Structural Insights into DMRTA1-DNA Binding Motifs via X-ray Crystallography*
**作者**：Yamamoto, T. et al.
**摘要**：通过X射线晶体学解析了重组人DMRTA1蛋白的DNA结合结构域，揭示了其与特定TATA-box序列的相互作用机制，为理解其在基因转录调控中的作用提供结构基础。
3. **文献名称**：*DMRTA1 Overexpression in Glioblastoma: Implications for Tumor Proliferation and Therapeutic Resistance*
**作者**：Zhang, L. et al.
**摘要**：研究发现胶质母细胞瘤中重组DMRTA1蛋白异常高表达，并通过调控细胞周期蛋白Cyclin D1加速肿瘤增殖，提示其作为潜在治疗靶点的可能性。
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**注**：以上文献为示例性内容，实际研究中建议通过PubMed或Google Scholar等平台以关键词“DMRTA1”、“recombinant protein”检索最新实证研究。
Recombinant human DMRTA1 (Doublesex and mab-3 related transcription factor A1) is a protein engineered to study the biological roles of the native DMRTA1 gene. DMRTA1 belongs to the DM domain-containing transcription factor family, known for regulating developmental processes, including neurogenesis, cell differentiation, and organogenesis. It shares structural homology with conserved sex-determination genes in other species, such as *Drosophila* Doublesex and *C. elegans* MAB-3. but its human ortholog is particularly implicated in brain development, notably in cortical neurogenesis and neural stem cell regulation.
The recombinant protein is typically produced using expression systems like *E. coli* or mammalian cells, followed by purification to retain functional integrity. Researchers utilize it to investigate DMRTA1's molecular mechanisms, such as DNA-binding specificity, protein-protein interactions, and downstream target gene activation. Aberrant DMRTA1 expression has been linked to neurodevelopmental disorders and certain cancers, making it a focus for understanding disease pathogenesis.
Structurally, DMRTA1 contains a conserved DM domain that facilitates DNA binding and dimerization, enabling transcriptional regulation. Studies suggest it cooperates with other factors (e.g., SOX proteins) to orchestrate gene networks during embryogenesis. Its recombinant form serves as a critical tool for functional assays, antibody production, and high-throughput screening to identify modulators of its activity, offering potential therapeutic insights.
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