| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DMBX1 |
| Uniprot No | Q8NFW5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-382aa |
| 氨基酸序列 | MQHYGVNGYS LHAMNSLSAM YNLHQQAAQQ AQHAPDYRPS VHALTLAERL AGCTFQDIIL EARYGSQHRK QRRSRTAFTA QQLEALEKTF QKTHYPDVVM RERLAMCTNL PEARVQVWFK NRRAKFRKKQ RSLQKEQLQK QKEAEGSHGE GKAEAPTPDT QLDTEQPPRL PGSDPPAELH LSLSEQSASE SAPEDQPDRE EDPRAGAEDP KAEKSPGADS KGLGCKRGSP KADSPGSLTI TPVAPGGGLL GPSHSYSSSP LSLFRLQEQF RQHMAATNNL VHYSSFEVGG PAPAAAAAAA AVPYLGVNMA PLGSLHCQSY YQSLSAAAAA HQGVWGSPLL PAPPAGLAPA SATLNSKTTS IENLRLRAKQ HAASLGLDTL PN |
| 分子量 | 41.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DMBX1蛋白的参考文献示例(文献为假设性模拟，仅供格式参考)：
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1. **文献名称**: *DMBX1: Molecular Cloning and Expression Analysis in Human Embryonic Brain Development*
**作者**: Tanaka K. et al.
**摘要**: 本研究成功克隆并表达了重组人DMBX1蛋白，发现其在人胚胎中脑发育阶段高表达，可能通过调控多巴胺能神经元分化参与早期神经发育。
2. **文献名称**: *Functional Characterization of Recombinant DMBX1 as a Transcriptional Repressor*
**作者**: Müller S. & Schmidt E.
**摘要**: 利用HEK293细胞表达带有His标签的DMBX1蛋白，证实其结合特定DNA序列并抑制靶基因转录，提示其作为转录抑制因子的功能机制。
3. **文献名称**: *Structural Insights into the DMBX1 Protein Interaction Network*
**作者**: Zhang L. et al.
**摘要**: 通过质谱和X射线晶体学分析，揭示重组DMBX1的三维结构及与多种神经发育相关蛋白(如OTX2)的相互作用，探讨其在调控基因网络中的作用。
4. **文献名称**: *Conservation and Divergence of DMBX1 Function in Zebrafish and Human*
**作者**: Kawakami Y. & Patel N.
**摘要**: 比较物种间DMBX1功能，发现重组人DMBX1在斑马鱼模型中可部分挽救同源基因敲除导致的视顶盖发育缺陷，表明其功能在进化中的保守性。
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注：以上文献为示例性内容，实际研究中需依据真实文献引用。如需具体论文，建议通过PubMed或Google Scholar以“DMBX1 recombinant protein”等关键词检索。
Recombinant human DMBX1 protein is a genetically engineered version of the diencephalon/mesencephalon homeobox protein 1. a transcription factor belonging to the homeodomain protein family. DMBX1 is encoded by the *DMBX1* gene and plays critical roles in early brain development, particularly in the patterning of the midbrain and forebrain regions. It regulates gene expression by binding to specific DNA sequences via its conserved homeodomain, a 60-amino acid motif that forms a helix-turn-helix structure. DMBX1 is implicated in neuronal differentiation, maintenance of precursor cells, and regional identity specification during embryogenesis.
The recombinant form is typically produced in *E. coli* or mammalian expression systems, enabling studies on its molecular functions without isolating it from native tissues. Researchers use it to investigate DNA-binding kinetics, protein-protein interactions, and regulatory networks in neurodevelopment. Its applications extend to modeling congenital brain disorders, neurodevelopmental defects, or neurodegenerative diseases linked to DMBX1 dysregulation. Additionally, it serves as a tool for exploring tissue engineering strategies targeting brain repair. Studies often include epitope tags (e.g., His-tag) for purification and tracking. Despite progress, the full spectrum of DMBX1’s interaction partners and post-translational modifications remains under exploration, highlighting the importance of recombinant protein tools in advancing this field.
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