| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DMAP1 |
| Uniprot No | Q9NPF5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-467aa |
| 氨基酸序列 | MATGADVRDI LELGGPEGDA ASGTISKKDI INPDKKKSKK SSETLTFKRP EGMHREVYAL LYSDKKDAPP LLPSDTGQGY RTVKAKLGSK KVRPWKWMPF TNPARKDGAM FFHWRRAAEE GKDYPFARFN KTVQVPVYSE QEYQLYLHDD AWTKAETDHL FDLSRRFDLR FVVIHDRYDH QQFKKRSVED LKERYYHICA KLANVRAVPG TDLKIPVFDA GHERRRKEQL ERLYNRTPEQ VAEEEYLLQE LRKIEARKKE REKRSQDLQK LITAADTTAE QRRTERKAPK KKLPQKKEAE KPAVPETAGI KFPDFKSAGV TLRSQRMKLP SSVGQKKIKA LEQMLLELGV ELSPTPTEEL VHMFNELRSD LVLLYELKQA CANCEYELQM LRHRHEALAR AGVLGGPATP ASGPGPASAE PAVTEPGLGP DPKDTIIDVV GAPLTPNSRK RRESASSSSS VKKAKKP |
| 分子量 | 52.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DMAP1蛋白的3篇模拟参考文献示例，基于合理推测与研究领域常见方向整理：
1. **文献名称**：*"Recombinant Human DMAP1 Expression and Interaction with DNMT1 in Epigenetic Silencing"*
**作者**：Rouse, J., et al.
**摘要**：研究通过大肠杆菌系统成功表达重组人DMAP1蛋白，并利用免疫共沉淀证实其与DNA甲基转移酶(DNMT1)的体外结合，揭示了DMAP1在基因沉默中的调控作用。
2. **文献名称**：*"Structural Insights into Human DMAP1-TIP60 Complex by X-ray Crystallography"*
**作者**：Zhang, L., et al.
**摘要**：通过重组表达获得高纯度DMAP1蛋白，结合X射线晶体学解析其与TIP60组蛋白乙酰转移酶的复合物结构，阐明了其在染色质修饰中的分子机制。
3. **文献名称**：*"DMAP1 Depletion Disrupts Cell Cycle Progression via p53-Dependent Pathways"*
**作者**：Thompson, M., et al.
**摘要**：利用重组DMAP1进行体外功能回补实验，发现其缺失导致细胞周期停滞于G1期，且通过调控p53信号通路影响细胞增殖与凋亡。
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**注**：以上文献为示例性内容，实际引用时请通过权威数据库(如PubMed、Web of Science)查询真实文献。如需进一步协助定位具体论文，可提供更多研究背景信息。
DMAp1 (DNA methyltransferase 1-associated protein 1) is a multifunctional nuclear protein involved in epigenetic regulation and chromatin remodeling. It was initially identified through its interaction with DNA methyltransferase 1 (DNMT1), a key enzyme maintaining DNA methylation patterns during replication. Human DMAP1 contains 467 amino acids and features distinct domains for binding DNMT1. histones, and other chromatin-modifying complexes like the TIP60 acetyltransferase complex. It plays dual roles in gene silencing and activation by bridging DNMT1-mediated DNA methylation and histone acetylation-dependent transcriptional activation.
As a component of the replication-dependent chromatin assembly machinery, DMAP1 contributes to heterochromatin formation, cell cycle progression, and DNA damage response. Studies link its dysfunction to carcinogenesis and developmental disorders, as abnormal DMAP1 expression disrupts DNA methylation homeostasis and genomic stability. Recombinant human DMAP1 protein is typically expressed in *E. coli* or mammalian systems with tags (e.g., GST, His-tag) for affinity purification. This engineered protein enables *in vitro* studies of its interactions with DNMT1. histones, or other partners, as well as biochemical assays to dissect its role in nucleosome assembly, chromatin dynamics, and methylation-related pathways. Its availability accelerates research into epigenetic therapeutics targeting DMAP1-associated networks in diseases like cancer.
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