| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DMRT1 |
| Uniprot No | Q9Y5R6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-373aa |
| 氨基酸序列 | MPNDEAFSKPSTPSEAPHAPGVPPQGRAGGFGKASGALVGAASGSSAGGSSRGGGSGSGASDLGAGSKKSPRLPKCARCRNHGYASPLKGHKRFCMWRDCQCKKCNLIAERQRVMAAQVALRRQQAQEEELGISHPIPLPSAAELLVKRENNGSNPCLMTECSGTSQPPPASVPTTAASEGRMVIQDIPAVTSRGHVENTPDLVSDSTYYSSFYQPSLFPYYNNLYNCPQYSMALAADSASGEVGNPLGGSPVKNSLRGLPGPYVPGQTGNQWQMKNMENRHAMSSQYRMHSYYPPPSYLGQSVPQFFTFEDAPSYPEARASVFSPPSSQDSGLVSLSSSSPISNKSTKAVLECEPASEPSSFTVTPVIEEDE |
| 分子量 | 66.77 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DMRT1蛋白的3-4条参考文献示例(注：部分文献信息为模拟概括，实际引用请核对真实数据库)：
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1. **文献名称**：*"Recombinant human DMRT1 binds DNA through a conserved zinc-finger domain to regulate sex-specific genes"*
**作者**：Smith J, Smith B, et al.
**摘要**：本研究利用大肠杆菌表达系统成功制备了重组人DMRT1蛋白，并通过电泳迁移率实验(EMSA)证明其特异性结合多个下游靶基因的DNA序列(如SOX9)，揭示了DMRT1通过保守锌指结构域调控性别分化相关基因的分子机制。
2. **文献名称**：*"Structural and functional analysis of recombinant human DMRT1 in testicular development"*
**作者**：Zhang H, Li Y, et al.
**摘要**：通过真核表达系统(昆虫细胞)获得高纯度重组人DMRT1蛋白，结合X射线晶体学解析其DNA结合域结构，证实其通过二聚化形成特异性识别基序，并激活睾丸发育相关通路。
3. **文献名称**：*"DMRT1-mediated transcriptional activation requires post-translational modifications of the recombinant protein"*
**作者**：Kobayashi T, et al.
**摘要**：研究探讨了重组人DMRT1蛋白的翻译后修饰(如磷酸化)对其功能的影响，发现修饰后的蛋白在体外细胞模型中显著增强对靶基因启动子的激活能力。
4. **文献名称**：*"Efficient production of functional human DMRT1 in a mammalian expression system and its role in germ cell differentiation"*
**作者**：Krentz AD, et al.
**摘要**：通过哺乳动物细胞(HEK293)表达重组人DMRT1蛋白，结合RNA-seq和ChIP-seq分析，证明其直接调控生殖细胞分化的关键基因，并应用于体外模型验证其功能。
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**提示**：实际研究中建议通过PubMed或Google Scholar以关键词“recombinant human DMRT1 protein”检索最新文献，或参考经典论文(如涉及DMRT1结构/功能的团队：Raymond, C.S., Murphy, M.W.等)。
DMRT1 (Doublesex and Mab-3 Related Transcription Factor 1) is a conserved zinc finger transcription factor critical for vertebrate sexual development and maintenance of gonadal function. In humans, it maps to chromosome 9p24.3 and plays a pivotal role in testis determination, spermatogenesis, and male germ cell survival. The protein contains a DMA domain (Doublesex/Mab-3 DNA-binding domain) that enables sequence-specific DNA binding and interaction with chromatin modifiers. DMRT1 regulates sex differentiation by controlling downstream targets such as SOX9 and AMH, while suppressing female pathway genes like FOXL2. Its dosage sensitivity is evident in disorders of sexual development (DSD), where hemizygous deletions are linked to 46.XY gonadal dysgenesis.
Recombinant human DMRT1 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to study its molecular mechanisms, post-translational modifications, and protein-protein interactions. It serves as a tool for investigating gonad development, germ cell maturation, and sex reversal syndromes. Researchers utilize purified DMRT1 to analyze DNA-binding kinetics, epigenetic regulation, and its role in mitigating germ cell tumors. The recombinant form retains functional domains required for dimerization, nuclear localization, and transcriptional activation/repression, enabling in vitro assays and disease modeling. Current studies also explore its therapeutic potential in fertility disorders and sexual dimorphism-related cancers.
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