| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DMGDH |
| Uniprot No | Q9UI17 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-866aa |
| 氨基酸序列 | MLRPGAQLLRGLLLRSCPLQGSPGRPRSVCGREGEEKPPLSAETQWKDRAETVIIGGGCVGVSLAYHLAKAGMKDVVLLEKSELTAGSTWHAAGLTTYFHPGINLKKIHYDSIKLYEKLEEETGQVVGFHQPGSIRLATTPVRVDEFKYQMTRTGWHATEQYLIEPEKIQEMFPLLNMNKVLAGLYNPGDGHIDPYSLTMALAAGARKCGALLKYPAPVTSLKARSDGTWDVETPQGSMRANRIVNAAGFWAREVGKMIGLEHPLIPVQHQYVVTSTISEVKALKRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKVQDSWVTNGVPPGFGKELFESDLDRIMEHIKAAMEMVPVLKKADIINVVNGPITYSPDILPMVGPHQGVRNYWVAIGFGYGIIHAGGVGKYLSDWILHGEPPFDLIELDPNRYGKWTTTQYTEAKARESYGFNNIVGYPKEERFAGRPTQRVSGLYQRLESKCSMGFHAGWEQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVAVTDLSPFGKFNIKGQDSIRLLDHLFANVIPKVGFTNISHMLTPKGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEEAVKGGYDVEIKNITDELGVLGVAGPQARKVLQKLTSEDLSDDVFKFLQTKSLKVSNIPVTAIRISYTGELGWELYHRREDSVALYDAIMNAGQEEGIDNFGTYAMNALRLEKAFRAWGLEMNCDTNPLEAGLEYFVKLNKPADFIGKQALKQIKAKGLKRRLVCLTLATDDVDPEGNESIWYNGKVVGNTTSGSYSYSIQKSLAFAYVPVQLSEVGQQVEVELLGKNYPAVIIQEPLVLTEPTRNRLQKKGGKDKT |
| 分子量 | 96.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人DMGDH蛋白的参考文献示例(内容基于文献常见方向构建，非真实文献)：
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1. **文献名称**: *"Heterologous Expression and Functional Analysis of Recombinant Human DMGDH in Insect Cells"*
**作者**: Zhang L. et al.
**摘要**: 该研究利用杆状病毒-昆虫细胞系统表达重组人DMGDH蛋白，并证实其具有催化二甲基甘氨酸脱氢的活性，为研究DMGDH酶动力学及缺陷相关疾病提供了工具。
2. **文献名称**: *"Structural Insights into Human DMGDH by Cryo-EM: Implications for Substrate Binding and Genetic Mutations"*
**作者**: Thompson R.J. et al.
**摘要**: 通过冷冻电镜解析了重组人DMGDH蛋白的高分辨率结构，揭示了其辅因子结合域和底物通道特征，解释了致病突变干扰酶活性的分子机制。
3. **文献名称**: *"A Recombinant DMGDH Assay for Diagnosis of Inborn Metabolic Disorders"*
**作者**: Wang Y. et al.
**摘要**: 开发了一种基于重组人DMGDH蛋白的功能检测方法，用于快速诊断因DMGDH缺乏导致的代谢疾病，验证了其在临床样本中的应用潜力。
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以上内容概括了重组DMGDH在表达系统、结构研究和临床诊断中的典型研究方向。如需真实文献，建议通过PubMed或Google Scholar以关键词“recombinant human DMGDH”检索。
Recombinant human dimethylglycine dehydrogenase (DMGDH) is a mitochondrial enzyme critical in choline metabolism. It catalyzes the oxidative demethylation of dimethylglycine (DMG) to sarcosine, producing hydrogen peroxide and 5.10-methylene-tetrahydrofolate. This reaction links choline catabolism to the folate cycle, influencing one-carbon metabolism essential for nucleotide synthesis and epigenetic regulation. DMGDH dysfunction is linked to rare inborn errors, causing elevated DMG and reduced sarcosine levels, with phenotypes ranging from cardiomyopathy to muscle weakness.
Recombinant DMGDH proteins are engineered using expression systems like E. coli or mammalian cells, often tagged for purification. They retain enzymatic activity, enabling structural studies, inhibitor screening, and disease modeling. Research focuses on understanding its redox cofactor (FAD) dependency, substrate specificity, and role in metabolic disorders. Recent studies also explore its potential as a biomarker for mitochondrial dysfunction or metabolic syndrome. The recombinant protein aids in elucidating molecular mechanisms of DMGDH deficiencies and developing therapeutic interventions targeting choline metabolism pathways.
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