| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DMRT2 |
| Uniprot No | Q9Y5R5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-561aa |
| 氨基酸序列 | MADPQAGSAA GDWEIDVESL ELEEDVCGAP RSTPPGPSPP PADGDCEDDE DDDGVDEDAE EEGDGEEAGA SPGMPGQPEQ RGGPQPRPPL APQASPAGTG PRERCTPAGG GAEPRKLSRT PKCARCRNHG VVSCLKGHKR FCRWRDCQCA NCLLVVERQR VMAAQVALRR QQATEDKKGL SGKQNNFERK AVYQRQVRAP SLLAKSILEG YRPIPAETYV GGTFPLPPPV SDRMRKRRAF ADKELENIML EREYKEREML ETSQAAALFL PNRMVPGPDY NSYKSAYSPS PVEPPSKDFC NFLPTCLDLT MQYSGSGNME LISSNVSVAT TYRQYPLSSR FLVWPKCGPI SDTLLYQQCL LNATTSVQAL KPGASWDLKG ARVQDGLSAE QDMMPSKLEG SLVLPHTPEI QTTRSDLQGH QAVPERSAFS PPRRNFSPIV DTDSLAAQGH VLTKISKENT RHPLPLRHNP FHSLFQQTLT DKSGPELKTP FVKEAFEETP KKHRECLVKD NQKYTFTIDR CAKDLFVAKQ VGTKLSVNEP LSFSVESILK RPSSAITRVS Q |
| 分子量 | 61.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DMRT2蛋白的参考文献摘要示例(注：以下文献为示例性概括，实际引用需核实具体论文信息)：
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1. **《Functional characterization of recombinant human DMRT2 in developmental regulation》**
- **作者**: Smith A, et al.
- **摘要**: 本研究通过原核系统成功表达并纯化了重组人DMRT2蛋白，利用体外DNA结合实验证实其特异性地结合生殖细胞发育相关基因的启动子区域，揭示其可能在早期胚胎分化中调控靶基因表达。
2. **《Structural insights into the DNA-binding domain of human DMRT2》**
- **作者**: Zhang L, et al.
- **摘要**: 作者通过X射线晶体学解析了重组人DMRT2蛋白DNA结合域的三维结构，揭示了其锌指模体与DNA相互作用的分子机制，为理解DMRT家族蛋白的功能提供了结构基础。
3. **《Recombinant DMRT2 regulates sex-specific gene networks in vitro》**
- **作者**: Tanaka K, et al.
- **摘要**: 该研究利用哺乳动物细胞系统表达重组DMRT2.结合RNA-seq分析发现其调控一系列性别分化相关基因的转录活性，提示DMRT2可能参与人类性腺发育的分子调控。
4. **《A novel purification strategy for recombinant human DMRT2 and its functional analysis》**
- **作者**: Patel R, et al.
- **摘要**: 开发了一种基于亲和层析的高效重组DMRT2蛋白纯化方法，并证明纯化蛋白在体外能够形成同源二聚体，增强对特定靶序列的结合能力，为后续功能研究提供了可靠工具。
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如需具体文献，建议通过PubMed或Web of Science以关键词“recombinant human DMRT2”检索最新研究。
DMRT2 (Doublesex and Mab-3 Related Transcription Factor 2) is a member of the evolutionarily conserved DMRT protein family, characterized by a zinc finger-like DNA-binding domain called the DM domain. This protein plays critical roles in vertebrate embryonic development, particularly in somitogenesis and central nervous system patterning. In humans, DMRT2 is located on chromosome 9p24.3 and functions as a transcriptional regulator involved in cell fate determination and tissue morphogenesis. Unlike its homolog DMRT1. which is strongly associated with sex determination, DMRT2’s primary functions extend to somitomeres segmentation and myogenesis coordination during early embryogenesis. Studies in model organisms reveal that DMRT2 deficiency leads to disrupted somitogenesis, vertebral malformations, and impaired muscle development.
Recombinant human DMRT2 protein is engineered using expression systems like *E. coli* or mammalian cells to produce functional protein for research applications. It enables mechanistic studies of DMRT2’s interaction with target DNA sequences, co-regulatory proteins (e.g., Tbx6. Mesp2), and signaling pathways (Notch, Wnt). Its recombinant form is crucial for *in vitro* assays, structural analyses, and disease modeling related to developmental disorders. Emerging evidence also links aberrant DMRT2 expression to cancers and congenital scoliosis, highlighting its broader biomedical relevance. By providing a purified, bioactive version of this regulatory protein, researchers can explore its therapeutic potential and role in regenerative medicine strategies.
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