| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DMRT3 |
| Uniprot No | Q9NQL9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-472aa |
| 氨基酸序列 | MNGYGSPYLY MGGPVSQPPR APLQRTPKCA RCRNHGVLSW LKGHKRYCRF KDCTCEKCIL IIERQRVMAA QVALRRQQAN ESLESLIPDS LRALPGPPPP GDAVAAPQPP PASQPSQPQP PRPAAELAAA AALRWTAEPQ PGALQAQLAK PDLTEERLGD GKSADNTEVF SDKDTDQRSS PDVAKSKGCF TPESPEIVSV EEGGYAVQKN GGNPESRPDS PKCHAEQNHL LIEGPSGTVS LPFSLKANRP PLEVLKKIFP NQKPTVLELI LKGCGGDLVS AVEVLLSSRS SVTGAERTSA EPESLALPSN GHIFEHTLSS YPISSSKWSV GSAFRVPDTL RFSADSSNVV PSPLAGPLQP PFPQPPRYPL MLRNTLARSQ SSPFLPNDVT LWNTMTLQQQ YQLRSQYVSP FPSNSTSVFR SSPVLPARAT EDPRISIPDD GCPFVSKQSI YTEDDYDERS DSSDSRTLNT SS |
| 分子量 | 51.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DMRT3蛋白的3篇示例参考文献(注：部分文献为假设性示例，实际研究需通过学术数据库核实)：
1. **《Functional analysis of human DMRT3 in motor neuron development》**
- 作者：Zhang L, et al.
- 摘要：研究通过重组表达人DMRT3蛋白，发现其在小鼠模型中对脊髓运动神经元的发育和回路形成具有调控作用，可能与步态协调相关。
2. **《Crystal structure of the DMRT3 DNA-binding domain reveals dimerization mechanism》**
- 作者：Kawarai S, et al.
- 摘要：解析重组人DMRT3蛋白的DNA结合域晶体结构，揭示其通过二聚化与靶基因启动子结合，为性别分化相关基因调控提供结构基础。
3. **《DMRT3 knockout in human iPSC-derived neurons impairs synaptic function》**
- 作者：Garcia-Ruiz A, et al.
- 摘要：利用CRISPR技术构建人诱导多能干细胞(iPSC)的DMRT3敲除模型，发现重组DMRT3蛋白回补可恢复神经元突触传递功能，提示其在神经系统疾病中的潜在作用。
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**提示**：实际文献建议通过PubMed/Google Scholar以关键词"recombinant human DMRT3"或"DMRT3 protein function"检索，重点关注近年涉及蛋白表达纯化、功能验证或疾病机制的研究。
DMRT3 (Doublesex and Mab-3 Related Transcription Factor 3) is a member of the DMRT gene family, which plays conserved roles in sex determination, gonadal development, and neuronal circuitry regulation across vertebrates. The protein contains a characteristic zinc finger-like DM domain that enables sequence-specific DNA binding and transcriptional regulation. In humans, DMRT3 is notably expressed in the developing central nervous system and gonads. Studies in model organisms, particularly horses and mice, highlight its critical function in coordinating locomotor rhythm by regulating spinal cord interneuron differentiation.
Recombinant human DMRT3 protein is engineered through heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies. Its production facilitates research into molecular mechanisms underlying neuronal development, sexual dimorphism, and potential disorders like reproductive anomalies or movement coordination deficits. Researchers utilize purified DMRT3 to analyze DNA-protein interactions, cellular signaling pathways, and gene expression networks. Recent explorations also link DMRT3 variants to neurodevelopmental conditions, emphasizing its therapeutic relevance. Challenges remain in maintaining structural stability during recombinant production and elucidating post-translational modifications affecting its regulatory activity. Ongoing work aims to refine expression protocols and define its interplay with cofactors in tissue-specific contexts.
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