| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNAI2 |
| Uniprot No | Q9GZS0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-593aa |
| 氨基酸序列 | MEIVYVYVKKRSEFGKQCNFSDRQAELNIDIMPNPELAEQFVERNPVDTGIQCSISMSEHEANSERFEMETRGVNHVEGGWPKDVNPLELEQTIRFRKKVEKDENYVNAIMQLGSIMEHCIKQNNAIDIYEEYFNDEEAMEVMEEDPSAKTINVFRDPQEIKRAATHLSWHPDGNRKLAVAYSCLDFQRAPVGMSSDSYIWDLENPNKPELALKPSSPLVTLEFNPKDSHVLLGGCYNGQIACWDTRKGSLVAELSTIESSHRDPVYGTIWLQSKTGTECFSASTDGQVMWWDIRKMSEPTEVVILDITKKEQLENALGAISLEFESTLPTKFMVGTEQGIVISCNRKAKTSAEKIVCTFPGHHGPIYALQRNPFYPKNFLTVGDWTARIWSEDSRESSIMWTKYHMAYLTDAAWSPVRPTVFFTTRMDGTLDIWDFMFEQCDPTLSLKDNGCLIACGSQLGTTTLLEVSPGLSTLQRNEKNVASSMFERETRREKILEARHREMRLKEKGKAEGRDEEQTDEELAVDLEALVSKAEEEFFDIIFTELKKKEADAIKLTPVPQQPSPEEDQVVEEGEEAAGEEGDEEVEEDLA |
| 分子量 | 90.97 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DNAI2蛋白的3篇参考文献示例(内容为虚构，仅作参考)：
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1. **文献名称**：*Cloning and Functional Characterization of Recombinant Human DNAI2 in Ciliary Dysfunction*
**作者**：Smith A, et al.
**摘要**：本研究首次克隆了人DNAI2基因，并通过哺乳动物表达系统成功获得重组蛋白，验证了其在纤毛组装中的关键作用，为原发性纤毛运动障碍(PCD)的分子机制提供了新见解。
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2. **文献名称**：*Structural Analysis of DNAI2 Mutants Associated with Primary Ciliary Dyskinesia*
**作者**：Chen L, et al.
**摘要**：通过体外重组表达人DNAI2蛋白及其常见致病突变体，结合冷冻电镜技术，揭示了突变导致蛋白结构稳定性丧失，进而影响纤毛内动力蛋白复合体的功能。
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3. **文献名称**：*Expression and Purification of Recombinant DNAI2 for Antibody Development*
**作者**：Wang Y, et al.
**摘要**：利用大肠杆菌系统高效表达可溶性DNAI2蛋白，优化纯化条件后制备出高特异性抗体，为PCD的临床诊断提供了可靠工具。
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4. **文献名称**：*DNAI2 Knockout Models Reveal Its Role in Sperm Motility*
**作者**：Johnson R, et al.
**摘要**：通过重组DNAI2蛋白回补实验，在基因敲除小鼠模型中证实其调控精子鞭毛运动的机制，为男性不育症研究提供了理论基础。
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注：实际文献需通过PubMed、Web of Science等平台以“DNAI2”、“recombinant protein”、“ciliary dysfunction”等关键词检索获取。
Recombinant human DNAI2 (dynein axonemal intermediate chain 2) protein is a genetically engineered version of the native DNAI2 protein, which plays a critical role in the structure and function of motile cilia and flagella. DNAI2 is a component of the dynein motor complex responsible for generating the mechanical force required for ciliary and flagellar movement. It is specifically expressed in tissues with motile cilia, such as respiratory epithelia and sperm flagella. Mutations in the DNAI2 gene are linked to primary ciliary dyskinesia (PCD), a rare genetic disorder characterized by impaired mucociliary clearance, chronic respiratory infections, and infertility.
The recombinant protein is typically produced using expression systems like *E. coli* or mammalian cell lines, enabling large-scale purification for research and therapeutic applications. Its production allows scientists to study DNAI2’s molecular interactions, structural properties, and the functional impact of disease-associated mutations. Recombinant DNAI2 is also used to generate antibodies, develop diagnostic assays for PCD, and explore targeted therapies to restore ciliary function. By serving as a standardized tool, it accelerates investigations into ciliary biology and the molecular basis of ciliopathies.
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