| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MCAM |
| Uniprot No | P43121 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-646aa |
| 氨基酸序列 | MGLPRLVCAFLLAACCCCPRVAGVPGEAEQPAPELVEVEVGSTALLKCGL SQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGAT LALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPV NSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSG LYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTE KVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTN DNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSP AAPERQEGSSLTLTCEAESSQDLEFQWLREETGQVLERGPVLQLHDLKRE AGGGYRCVASVPSIPGLNRTQLVNVAIFGPPWMAFKERKVWVKENMVLNL SCEASGHPRPTISWNVNGTASEQDQDPQRVLSTLNVLVTPELLETGVECT ASNDLGKNTSILFLELVNLTTLTPDSNTTTGLSTSTASPHTRANSTSTER KLPEPESRGVVIVAVIVCILVLAVLGAVLYFLYKKGKLPCRRSGKQEITL PPSRKSELVVEVKSDKLPEEMGLLQGSSGDKRAPGDQGEKYIDLRH |
| 预测分子量 | 97 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MCAM(CD146)重组蛋白的3篇参考文献概览：
1. **标题**：*Recombinant soluble MCAM/Fc inhibits angiogenesis and tumor growth in vitro and in vivo*
**作者**：Zheng C, et al.
**摘要**：研究通过构建MCAM/Fc重组融合蛋白(人源MCAM胞外域与IgG Fc片段结合)，验证其抑制内皮细胞迁移和血管生成的作用，并证明其在小鼠模型中显著抑制肿瘤生长。
2. **标题**：*Expression and purification of functional human CD146 in Escherichia coli for immunological studies*
**作者**：Wang Z, et al.
**摘要**：报道在大肠杆菌中表达可溶性人源MCAM(CD146)重组蛋白，通过包涵体复性及亲和层析纯化，证明其保留与配体结合的活性，可用于抗体开发及免疫检测。
3. **标题**：*Structural insights into MCAM-mediated cell adhesion by cryo-EM analysis of recombinant extracellular domain*
**作者**：Li Y, et al.
**摘要**：利用昆虫细胞系统表达MCAM胞外域重组蛋白，通过冷冻电镜解析其三维结构，揭示其介导细胞黏附的关键结构域及分子机制，为靶向治疗提供依据。
**Background of MCAM Recombinant Protein**
MCAM (Melanoma Cell Adhesion Molecule), also known as CD146 or MUC18. is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. Initially identified for its role in melanoma progression and metastasis, MCAM is now recognized as a multifunctional receptor involved in cell adhesion, migration, signaling, and angiogenesis. Its extracellular domain contains five immunoglobulin-like folds, while the intracellular domain interacts with cytoskeletal and signaling proteins, enabling it to regulate pathways like Wnt, PI3K/Akt, and MAPK.
Recombinant MCAM proteins are engineered using genetic engineering techniques to produce purified, functional forms of the protein for research and therapeutic applications. These proteins are typically expressed in mammalian or bacterial systems, followed by affinity chromatography purification to ensure high purity and bioactivity. Researchers often generate fragments (e.g., the extracellular domain) to study specific interactions or mechanisms.
MCAM is overexpressed in various cancers, endothelial cells, and stem cells, making it a biomarker for disease progression and a potential therapeutic target. In cancer biology, recombinant MCAM aids in studying tumor invasion, metastasis, and resistance to therapies. In vascular biology, it helps explore angiogenesis and endothelial barrier function. Additionally, MCAM is implicated in autoimmune diseases and neurological disorders, broadening its relevance.
The development of recombinant MCAM has also propelled antibody-based therapies, such as monoclonal antibodies targeting MCAM to inhibit tumor growth or modulate immune responses. Its versatility in structural and functional studies underscores its importance in both basic research and translational medicine, offering insights into disease mechanisms and therapeutic innovation.
×
