Recombinant Human CAMK4 protein

中文名： 钙/钙调蛋白依赖性蛋白激酶IV型(CAMK4)重组蛋白
别名： CAMK4；CAMK；CAMK-GR；CAMKIV；Calcium/calmodulin-dependent protein kinase type IV

货号: PA1000-7542

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	CAMK4
Uniprot No	Q16566
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-473aa
氨基酸序列	MGSSHHHHHHSSGLVPRGSHMGSHMLKVTVPSCSASSCSSVTASAAPGTA SLVPDYWIDGSNRDALSDFFEVESELGRGATSIVYRCKQKGTQKPYALKV LKKTVDKKIVRTEIGVLLRLSHPNIIKLKEIFETPTEISLVLELVTGGEL FDRIVEKGYYSERDAADAVKQILEAVAYLHENGIVHRDLKPENLLYATPA PDAPLKIADFGLSKIVEHQVLMKTVCGTPGYCAPEILRGCAYGPEVDMWS VGIITYILLCGFEPFYDERGDQFMFRRILNCEYYFISPWWDEVSLNAKDL VRKLIVLDPKKRLTTFQALQHPWVTGKAANFVHMDTAQKKLQEFNARRKL KAAVKAVVASSRLGSASSSHGSIQESHKASRDPSPIQDGNEDMKAIPEGE KIQGDGAQAAVKGAQAELMKVQALEKVKGADINAEEAPKMVPKAVEDGIK VADLELEEGLAEEKLKTVEEAAAPREGQGSSAVGFEVPQQDVILPEY
预测分子量	55 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于CAMK4重组蛋白的3篇参考文献示例(注：内容为模拟生成，建议通过学术数据库验证准确性)：

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1. **标题**：*"Expression and Purification of Recombinant CAMK4 in Escherichia coli for Functional Studies"*

**作者**：Tanaka M, et al.

**摘要**：研究报道了在大肠杆菌中高效表达和纯化重组CAMK4蛋白的方法，通过优化诱导条件和亲和层析技术获得高纯度蛋白，并验证其激酶活性依赖于钙/钙调蛋白的调控。

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2. **标题**：*"CAMK4-Mediated Phosphorylation of CREB in Neuronal Signaling Pathways"*

**作者**：Li X, Wang Y, Chen Z.

**摘要**：利用重组CAMK4蛋白进行体外激酶实验，证明其通过磷酸化转录因子CREB参与神经元突触可塑性和记忆形成的分子机制，为神经退行性疾病研究提供新靶点。

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3. **标题**：*"Structural Insights into CAMK4 Activation by Calcium Signaling"*

**作者**：Rodriguez S, et al.

**摘要**：通过X射线晶体学解析重组CAMK4蛋白的活化构象，揭示钙离子结合后其自抑制结构域的构象变化，阐明了激酶活性调控的结构基础。

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提示：建议通过PubMed或Google Scholar检索关键词“recombinant CAMK4 protein”获取最新文献，或结合具体研究方向(如疾病模型、信号通路)进一步筛选。

背景信息

**Background of CAMK4 Recombinant Protein**

Calcium/calmodulin-dependent protein kinase IV (CAMK4) is a serine/threonine kinase belonging to the Ca²⁺/calmodulin-dependent kinase (CAMK) family. It plays a critical role in calcium-mediated signaling pathways, regulating processes such as gene transcription, synaptic plasticity, immune responses, and neuronal survival. CAMK4 is activated by elevated intracellular Ca²⁺ levels, which promote its binding to calmodulin, leading to autophosphorylation and subsequent kinase activity. It phosphorylates transcription factors like CREB (cAMP response element-binding protein), facilitating their interaction with coactivators to modulate expression of genes involved in learning, memory, and inflammation.

Recombinant CAMK4 protein is engineered through molecular cloning, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., insect or mammalian cells) to ensure proper post-translational modifications. This purified protein retains enzymatic activity and structural integrity, making it a vital tool for *in vitro* studies. Researchers use it to dissect CAMK4-dependent signaling mechanisms, screen kinase inhibitors, or explore its interactions with downstream targets. Its applications extend to disease models, as CAMK4 dysregulation is implicated in neurological disorders (e.g., Alzheimer’s), autoimmune diseases, and cancer.

The availability of recombinant CAMK4 has accelerated drug discovery and mechanistic studies, offering insights into its therapeutic potential. However, challenges remain in mimicking native cellular environments *in vitro*, necessitating careful validation of experimental conditions. Overall, CAMK4 recombinant protein serves as a cornerstone for understanding calcium signaling networks and developing targeted therapies.