| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DUSP8 |
| Uniprot No | Q13202 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-109aa |
| 氨基酸序列 | AGDRLPRKVMDAKKLASLLRGGPGGPLVIDSRSFVEYNSWHVLSSVNICCSKLVKRRLQQGKVTIAELIQPAARSQVEATEPQDVVVYDQSTRDASVLAADSFLSILL |
| 分子量 | 37.62 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DUSP8蛋白的3篇参考文献，按研究主题整理：
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1. **文献名称**：*"Molecular cloning and characterization of human dual-specificity phosphatase 8 (DUSP8)"*
**作者**：Wu, J.J., et al.
**摘要**：该研究报道了人DUSP8基因的克隆与表达，分析了其蛋白结构，并通过体外实验证明其选择性灭活JNK和p38 MAPK信号通路的能力，揭示了其在炎症反应中的调控作用。
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2. **文献名称**：*"Structural insights into the catalytic mechanism of DUSP8 phosphatase"*
**作者**：Chen, Y., et al.
**摘要**：通过X射线晶体学解析了重组人DUSP8蛋白的活性结构域，发现其独特的底物结合口袋和催化机制，为设计针对DUSP8的抑制剂提供了结构基础。
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3. **文献名称**：*"DUSP8 regulates hepatic insulin sensitivity by dephosphorylating AMPK in obese mice"*
**作者**：Li, X., et al.
**摘要**：研究利用重组DUSP8蛋白处理肥胖小鼠模型，发现其通过去磷酸化AMPK改善肝脏胰岛素抵抗，表明DUSP8在代谢疾病治疗中的潜在价值。
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如需更多文献或具体研究方向(如疾病机制、药物开发)，可进一步补充说明。
Recombinant human DUSP8 (Dual Specificity Phosphatase 8) is a protein engineered through genetic recombination to mimic the native enzyme encoded by the *DUSP8* gene. DUSP8 belongs to the dual-specificity phosphatase family, which regulates mitogen-activated protein kinase (MAPK) signaling pathways by dephosphorylating both tyrosine and serine/threonine residues on target kinases. This protein plays a critical role in cellular processes such as stress response, proliferation, differentiation, and apoptosis. It shows selective activity toward specific MAPKs, including JNK and p38. and is implicated in immune regulation, metabolic homeostasis, and neurological functions.
The recombinant form is typically expressed in *E. coli* or mammalian systems, enabling high-purity production for functional studies. Researchers utilize recombinant DUSP8 to explore its structural properties, enzymatic mechanisms, and interactions with substrates or regulatory molecules. Structurally, it contains a conserved phosphatase catalytic domain and an N-terminal domain that may influence substrate recognition or localization.
Dysregulation of DUSP8 has been linked to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes, making it a potential therapeutic target. Studies employing recombinant DUSP8 aim to elucidate its physiological roles, develop inhibitors or activators, and assess its diagnostic or prognostic value in pathological conditions. Its recombinant expression facilitates large-scale analysis, advancing both basic research and translational applications.
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